{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:15:48Z","timestamp":1787652948022,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,3,28]],"date-time":"2011-03-28T00:00:00Z","timestamp":1301270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2011,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> Starchy seeds are an important food and a source of dietary ingredients in many countries. However, they suffer from extensive predation by bruchids (weevils) and other pests. \u03b1\u2010Amylase inhibitors are attractive candidates for the control of seed weevils, as these insects are highly dependent on starch as an energy source.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> A proteinaceous \u03b1\u2010amylase inhibitor from the seeds of <jats:italic>Achyranthes aspera<\/jats:italic> was identified, purified and characterised. In electrophoretic analysis, two prominent amylase inhibitor activity bands (AI1 and AI2) were detected. The inhibitor was purified 9.99\u2010fold with 1206.95 total amylase inhibitor units mg<jats:sup>\u22121<\/jats:sup> protein. The molecular weight of the purified inhibitor was around 6 kDa. The isolated \u03b1\u2010amylase inhibitor was found to be resistant to heat and proteolysis. Feeding analysis of <jats:italic>Callosobruchus maculatus<\/jats:italic> larvae on a diet containing seed powder of <jats:italic>A. aspera<\/jats:italic> revealed that survival of the larvae was severely affected, with the highest mortality rate occurring on the fifth day of feeding. The isolated inhibitor inhibited the majority of amylase isoforms of <jats:italic>C. maculatus, Tribolium confusum<\/jats:italic> and <jats:italic>Helicoverpa armigera<\/jats:italic> in electrophoretic analysis and solution assays.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> The information obtained in the present investigation could be useful for a genetic engineering approach that would make seeds resistant to storage pest infestations. 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