{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:11:52Z","timestamp":1760872312006,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,9,14]],"date-time":"2011-09-14T00:00:00Z","timestamp":1315958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> Four repetitive element sequence\u2010based polymerase chain reaction (rep\u2010PCR) methods, namely repetitive extragenic palindromic PCR (REP\u2010PCR), enterobacterial repetitive intergenic consensus PCR (ERIC\u2010PCR), polytrinucleotide (GTG)<jats:sub>5<\/jats:sub>\u2010PCR and BOX\u2010PCR, were evaluated for the molecular differentiation of 12 probiotic <jats:bold><jats:italic>Lactobacillus<\/jats:italic><\/jats:bold> strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep\u2010PCR methods and their combination (composite rep\u2010PCR) in the molecular typing of <jats:bold><jats:italic>Lactobacillus<\/jats:italic><\/jats:bold> strains based on a discriminatory index (<jats:italic>D<\/jats:italic>).<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Species\u2010specific and strain\u2010specific profiles were observed from rep\u2010PCR. From the numerical analysis of composite rep\u2010PCR, BOX\u2010PCR, (GTG)<jats:sub>5<\/jats:sub>\u2010PCR, REP\u2010PCR and ERIC\u2010PCR, <jats:italic>D<\/jats:italic> values of 0.9118, 0.9044, 0.8897, 0.8750 and 0.8529 respectively were obtained. Composite rep\u2010PCR analysis was the most discriminative method, with eight <jats:bold><jats:italic>Lactobacillus<\/jats:italic><\/jats:bold> strains, namely <jats:bold><jats:italic>L. brevis<\/jats:italic><\/jats:bold> ATCC 14869<jats:sup>T<\/jats:sup>, <jats:bold><jats:italic>L. reuteri<\/jats:italic><\/jats:bold> C 10, <jats:bold><jats:italic>L. reuteri<\/jats:italic><\/jats:bold> ATCC 23272<jats:sup>T<\/jats:sup>, <jats:bold><jats:italic>L. gallinarum<\/jats:italic><\/jats:bold> ATCC 33199<jats:sup>T<\/jats:sup>, <jats:bold><jats:italic>L. salivarius<\/jats:italic><\/jats:bold> ATCC 11741<jats:sup>T<\/jats:sup>, <jats:bold><jats:italic>L. salivarius<\/jats:italic><\/jats:bold> I 24, <jats:bold><jats:italic>L. panis<\/jats:italic><\/jats:bold> JCM 11053<jats:sup>T<\/jats:sup> and <jats:bold><jats:italic>L. panis<\/jats:italic><\/jats:bold> C 17, being differentiated at the strain level.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> Composite rep\u2010PCR analysis is potentially a useful fingerprinting method to discriminate probiotic <jats:bold><jats:italic>Lactobacillus<\/jats:italic><\/jats:bold> strains isolated from the gastrointestinal tract of chickens. Copyright \u00a9 2011 Society of Chemical Industry<\/jats:p>","DOI":"10.1002\/jsfa.4627","type":"journal-article","created":{"date-parts":[[2011,9,14]],"date-time":"2011-09-14T09:00:28Z","timestamp":1315990828000},"page":"660-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Discrimination of probiotic <b><i>Lactobacillus<\/i><\/b> strains for poultry by repetitive sequenced\u2010based PCR fingerprinting"],"prefix":"10.1002","volume":"92","author":[{"given":"Chin Mei","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chin 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