{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:57:43Z","timestamp":1789423063601,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,6,15]],"date-time":"2012-06-15T00:00:00Z","timestamp":1339718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2013,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND: A variety of lactose imprinted hydrogels were prepared and their binding properties were studied in comparison with blank non\u2010imprinted hydrogel. Methacrylamide and ethylene glycol dimethacrylate were used as functional monomer and cross\u2010linker, respectively. Dimethylsulfoxide was also applied as polymerisation solvent.<\/jats:bold><\/jats:p><jats:p><jats:bold>RESULTS: Different template\/monomer ratios were studied and the optimised imprinted hydrogel (MIP<jats:sub>2<\/jats:sub>), with a lactose\/methacrylamide ratio of 1:8, was selected in a rebinding test. In Scatchard analysis of MIP<jats:sub>2<\/jats:sub>\u2010lactose interactions, the dissociation constant and maximum binding sites were 0.33 mmol L<jats:sup>\u22121<\/jats:sup> and 67.76 \u00b5mol g<jats:sup>\u22121<\/jats:sup> hydrogel, respectively. The selectivity of MIP<jats:sub>2<\/jats:sub> for lactose in aqueous media was also evaluated in comparison with different mono\u2010 and disaccharides. The data showed that the affinity of MIP<jats:sub>2<\/jats:sub> for lactose is significantly higher than other saccharides. The imprinted hydrogel was finally used as a sorbent for separation of lactose from milk.<\/jats:bold><\/jats:p><jats:p><jats:bold>CONCLUSIONS: The results indicated that MIP<jats:sub>2<\/jats:sub>, as an optimised imprinted hydrogel, can effectively bind lactose and decrease its concentration in milk. Copyright \u00a9 2012 Society of Chemical Industry<\/jats:bold><\/jats:p>","DOI":"10.1002\/jsfa.5757","type":"journal-article","created":{"date-parts":[[2012,6,15]],"date-time":"2012-06-15T14:29:55Z","timestamp":1339770595000},"page":"304-309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Application of molecularly imprinted hydrogel for the preparation of lactose\u2010free milk"],"prefix":"10.1002","volume":"93","author":[{"given":"Farzin","family":"Hadizadeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Hassanpour Moghadam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed 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