{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:40:06Z","timestamp":1760866806715,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T00:00:00Z","timestamp":1340323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2013,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND: Brinjal is an important vegetable crop. Major crop loss of brinjal is due to insect attack. Insect\u2010resistant EE\u20101 brinjal has been developed and is awaiting approval for commercial release. Consumer health concerns and implementation of international labelling legislation demand reliable analytical detection methods for genetically modified (GM) varieties.<\/jats:bold><\/jats:p><jats:p><jats:bold>RESULTS: End\u2010point and real\u2010time polymerase chain reaction (PCR) methods were used to detect EE\u20101 brinjal. In end\u2010point PCR, primer pairs specific to 35S CaMV promoter, NOS terminator and nptII gene common to other GM crops were used. Based on the revealed 3\u2032 transgene integration sequence, primers specific for the event EE\u20101 brinjal were designed. These primers were used for end\u2010point single, multiplex and SYBR\u2010based real\u2010time PCR. End\u2010point single PCR showed that the designed primers were highly specific to event EE\u20101 with a sensitivity of 20 pg of genomic DNA, corresponding to 20 copies of haploid EE\u20101 brinjal genomic DNA. The limits of detection and quantification for SYBR\u2010based real\u2010time PCR assay were 10 and 100 copies respectively.<\/jats:bold><\/jats:p><jats:p><jats:bold>CONCLUSION: The prior development of detection methods for this important vegetable crop will facilitate compliance with any forthcoming labelling regulations. Copyright \u00a9 2012 Society of Chemical Industry<\/jats:bold><\/jats:p>","DOI":"10.1002\/jsfa.5764","type":"journal-article","created":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T16:15:07Z","timestamp":1340381707000},"page":"340-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection and identification of genetically modified EE\u20101 brinjal (<i>Solanum melongena<\/i>) by single, multiplex and SYBR<sup>\u00ae<\/sup> real\u2010time PCR"],"prefix":"10.1002","volume":"93","author":[{"given":"Rajashekhar V","family":"Ballari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asha","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lalitha R","family":"Gowda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1300\/J153v06n02_02"},{"volume-title":"Origin of Cultivated Plants, 1886","year":"1959","author":"De Candolle A","key":"e_1_2_7_3_1"},{"key":"e_1_2_7_4_1","unstructured":"FAO Agriculture database. 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