{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:36:38Z","timestamp":1760920598608,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,12,12]],"date-time":"2012-12-12T00:00:00Z","timestamp":1355270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:bold>Yersiniosis is one of the three leading foodborne zoonoses in Lithuania, and the incidence of 12.86 per 100\u2009000 population was the highest among <jats:styled-content style=\"fixed-case\">EU<\/jats:styled-content> member states in 2010. Contaminated pig carcasses and subsequently undercooked pig meat are considered to be the primary transmission vehicle of enteropathogenic <jats:italic>Y. enterocolitica<\/jats:italic> to consumers. With the aim of evaluating pigs as a possible source of human yersiniosis in Lithuania, this study investigated the genetic diversity of <jats:italic>Y. enterocolitica<\/jats:italic> isolated from pigs and human cases of yersiniosis. In addition, the antimicrobial resistance of selected isolates from both sources was compared<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:bold>In total, 83 <jats:italic>Y. enterocolitica<\/jats:italic> strains were characterised using pulsed field gel electrophoresis. Overall, 68% of <jats:italic>Y. enterocolitica<\/jats:italic> 4\/O:3 pulsotypes found in human clinical samples were identical to 81% of pulsotypes found in the pig production chain. <jats:italic>Yersinia enterocolitica<\/jats:italic> pulsotype <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> was confirmed as the dominant pulsotype in the pig production chain and was identical to nine of 19 <jats:italic>Y. enterocolitica<\/jats:italic> strains found in humans. All tested <jats:italic>Y. enterocolitica<\/jats:italic> 4\/O:3 strains were resistant to ampicillin and erythromycin and sensitive to ciprofloxacin. Of the strains studied, 5% were resistant to tetracycline and streptomycin<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:bold>This study showed that pigs may be the main source of human yersiniosis in Lithuania. In addition, <jats:italic>Y. enterocolitica<\/jats:italic> 4\/O:3 strains isolated from the pig production chain and from yersiniosis patients shared similar resistance to different antimicrobials.\u00a9 2012 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.5980","type":"journal-article","created":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T06:56:24Z","timestamp":1351752984000},"page":"1858-1862","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genetic diversity and antimicrobial resistance of <i>Yersinia enterocolitica<\/i> isolated from pigs and humans in Lithuania"],"prefix":"10.1002","volume":"93","author":[{"given":"Aleksandr","family":"Novoslavskij","sequence":"first","affiliation":[{"name":"Department of Food Safety and Quality, Faculty of Veterinary Medicine, Veterinary Academy Lithuanian University of Health Sciences  Til\u017e\u0117s 18 LT\u201047181 Kaunas Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Egl\u0117","family":"Kudirkien\u0117","sequence":"additional","affiliation":[{"name":"Department of Food Safety and Quality, Faculty of Veterinary Medicine, Veterinary Academy Lithuanian University of Health Sciences  Til\u017e\u0117s 18 LT\u201047181 Kaunas Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audron\u0117","family":"Marcinkut\u0117","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Dermatovenereology and Microbiology, Faculty of Medicine Vilnius University  M.K. \u010ciurlionio 21 LT\u201003101 Vilnius Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almina","family":"Bajori\u016bnien\u0117","sequence":"additional","affiliation":[{"name":"Tuberculosis and Infectious Diseases Hospital Vilnius University  Santari\u0161ki\u0173 Klinikos Birut\u0117s 1 LT\u201008117 Vilnius Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannu","family":"Korkeala","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine University of Helsinki  PO Box\u00a066 Agnes Sj\u00f6bergin katu 2 FI\u201000014 Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mindaugas","family":"Malakauskas","sequence":"additional","affiliation":[{"name":"Department of Food Safety and Quality, Faculty of Veterinary Medicine, Veterinary Academy Lithuanian University of Health Sciences  Til\u017e\u0117s 18 LT\u201047181 Kaunas Lithuania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)80028-8"},{"key":"e_1_2_6_3_1","first-page":"190","article-title":"The Community Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents, Antimicrobial Resistance and Foodborne Outbreaks in the European Union in 2006","volume":"130","year":"2007","journal-title":"EFSA J"},{"key":"e_1_2_6_4_1","first-page":"190","article-title":"The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food\u2010borne Outbreaks in 2010","volume":"2597","year":"2012","journal-title":"EFSA J"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(91)90007-C"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(96)01223-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00095.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46523-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268801005611"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46733-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0265"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2011.07.017"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2011.0012"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3674-3681.2005"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2003.00718.x"},{"key":"e_1_2_6_16_1","first-page":"14","article-title":"Revised biogrouping scheme of Yersinia enterocolitica","volume":"9","author":"Wauters G","year":"1987","journal-title":"Contrib Microbiol Immunol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1999.00625.x"},{"key":"e_1_2_6_18_1","unstructured":"CLSI (Clinical and Laboratory Standards Institute) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard \u2013 seventh edition.CLSI Document M7\u2010A7 p.\u00a01\u201364(2006)."},{"key":"e_1_2_6_19_1","unstructured":"CLSI (Clinical and Laboratory Standards Institute) Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement.CLSI Document M100\u2010S16 p.\u00a01\u2013188(2006)."},{"key":"e_1_2_6_20_1","unstructured":"DANMAP Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals food and humans in Denmark. [Online]. (2008).Available: http:\/\/danmap.org[10 January 2011]."},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.9.1903-1905.1990"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0251"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0461"},{"key":"e_1_2_6_24_1","first-page":"2913","article-title":"Iteman I and Carniel E","volume":"32","author":"Najdenski H","year":"1994","journal-title":"Efficient subtyping of pathogenic Y. enterocolitica strains by pulsed\u2010field gel electrophoresis. J Clin Microbiol"},{"key":"e_1_2_6_25_1","article-title":"Prevalence and genetic diversity of enteropathogenic Yersinia in pigs at farms and slaughter in Lithuania","author":"Novoslavskij A","year":"2012","journal-title":"Res Vet Sci"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.12.2423"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1140-1145.2000"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.10.008"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.9.1680"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-1-141"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00241-1"},{"key":"e_1_2_6_32_1","first-page":"39","article-title":"Occurrence of pathogenic strains of Yersinia enterocolitica in pigs and their antimicrobial resistance","volume":"52","author":"Simonova J","year":"2008","journal-title":"Bull Vet Inst Pulawy"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.7.1335"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2004.04.006"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.5980","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.5980","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.5980","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:18:55Z","timestamp":1760876335000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.5980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,12]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/jsfa.5980"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.5980","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"type":"print","value":"0022-5142"},{"type":"electronic","value":"1097-0010"}],"subject":[],"published":{"date-parts":[[2012,12,12]]},"assertion":[{"value":"2012-03-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}