{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:32:13Z","timestamp":1777530733922,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,7,26]],"date-time":"2013-07-26T00:00:00Z","timestamp":1374796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:bold>Plants have developed an efficient system of recognition that induces a complex network of signalling molecules such as salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>), jasmonic acid (<jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content>) and abscisic acid (<jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>) in case of a pathogenic infection. The use of specific and sensitive methods is mandatory for the analysis of compounds in these complex samples<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:bold>In this study a liquid chromatography\/electrospray ionisation tandem mass spectrometry method was developed and validated for the simultaneous quantification of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content> in <jats:italic>Coffea arabica<\/jats:italic> (L.) leaves in order to understand the role of these phytohormones in the signalling network involved in the coffee defence response against <jats:italic>Hemileia vastatrix<\/jats:italic>. The results showed that the method was specific, linear (<jats:italic>r<\/jats:italic> \u2265 0.99) in the range 0.125\u20131.00 \u00b5g <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> for <jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content> and 0.125\u20135.00 \u00b5g <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> for <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>, and precise (relative standard deviation \u226411%), and the limit of detection (0.010 \u00b5g g<jats:sup>\u22121<\/jats:sup> fresh weight) was adequate for quantifying these phytohormones in this type of matrix<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:bold>In comparison with healthy leaves, those infected with <jats:italic>H. vastatrix<\/jats:italic> (resistance reaction) displayed an increase in <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> level 24 h after inoculation, suggesting the involvement of an <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010dependent pathway in coffee resistance. \u00a9 2013 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.6288","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T10:55:06Z","timestamp":1372157706000},"page":"529-536","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["A liquid chromatography\/electrospray ionisation tandem mass spectrometry method for the simultaneous quantification of salicylic, jasmonic and abscisic acids in <i>Coffea arabica<\/i> leaves"],"prefix":"10.1002","volume":"94","author":[{"given":"Marta","family":"de S\u00e1","sequence":"first","affiliation":[{"name":"Agronomia Tropical, Instituto Superior de Agronomia (ISA) Universidade T\u00e9cnica de Lisboa (UTL) Lisboa Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o P","family":"Ferreira","sequence":"additional","affiliation":[{"name":"iMED, Faculdade de Farm\u00e1cia Universidade de Lisboa (UL) Lisboa Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vagner T","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o das Ferrugens do Cafeeiro (CIFC) BioTrop, Instituto de Investiga\u00e7\u00e3o Cient\u00edfica Tropical (IICT) Oeiras Portugal"},{"name":"Centro de Ci\u00eancias Agr\u00e1rias (CCA) Universidade Federal do Esp\u00edrito Santo (UFES) Alegre (ES) Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds","family":"Vilas\u2010Boas","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica (IBET) Esta\u00e7\u00e3o Agron\u00f3mica Nacional Apartado 12 P\u20102781\u2010901 Oeiras Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria C","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o das Ferrugens do Cafeeiro (CIFC) BioTrop, Instituto de Investiga\u00e7\u00e3o Cient\u00edfica Tropical (IICT) Oeiras Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria H","family":"Almeida","sequence":"additional","affiliation":[{"name":"Agronomia Tropical, Instituto Superior de Agronomia (ISA) Universidade T\u00e9cnica de Lisboa (UTL) Lisboa Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonor","family":"Guerra\u2010Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o das Ferrugens do Cafeeiro (CIFC) BioTrop, Instituto de Investiga\u00e7\u00e3o Cient\u00edfica Tropical (IICT) Oeiras Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria R","family":"Bronze","sequence":"additional","affiliation":[{"name":"iMED, Faculdade de Farm\u00e1cia Universidade de Lisboa (UL) Lisboa Portugal"},{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica (IBET) Esta\u00e7\u00e3o Agron\u00f3mica Nacional Apartado 12 P\u20102781\u2010901 Oeiras Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica (ITQB) Universidade Nova de Lisboa, Avenida da Rep\u00fablica Esta\u00e7\u00e3o Agron\u00f3mica Nacional P\u20102780\u2010157 Oeiras Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-008-9435-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.164"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3mb25569g"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-0473-9_9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.615"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-002-0866-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2209"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2008.04.041"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1085"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf062249r"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.03.007"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050884b"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2008.02.008"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1677-04202006000100010"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2004.00250.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-011-9925-9"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/pmpp.2002.0389"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2005.02.006"},{"key":"e_1_2_6_20_1","first-page":"8","article-title":"Commission Decision 2002\/657\/EC implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and interpretation of results","volume":"221","year":"2002","journal-title":"Off J Eur Commun"},{"key":"e_1_2_6_21_1","volume-title":"Water Quality\u2014Calibration and Evaluation of Analytical Methods and Estimation of Performance Characteristics\u2014Part 1: Statistical Evaluation of the Linear Calibration Function"},{"key":"e_1_2_6_22_1","unstructured":"Van TrijpJMPandRoosAH Model for the calculation of calibration curves.RIKILT Report 91.02 RIKILT\u2010DLO Wageningen (1991)."},{"key":"e_1_2_6_23_1","volume-title":"AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals","year":"2002"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1255\/ejms.220"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.11.017"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(94)85056-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2003.07.002"},{"key":"e_1_2_6_28_1","first-page":"743","article-title":"Chlorogenic acid content in coffee leaves: possible role in coffee leaf rust resistance","author":"Leit\u00e3o S","year":"2010","journal-title":"Proc. 23rd Int. Conf. on Coffee Science (ASIC)"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ers027"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027358423187"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-90162008000400005"},{"key":"e_1_2_6_32_1","first-page":"1036","article-title":"Differential induction of superoxide dismutase in Coffea Arabica\u2013Hemileia vastatrix interactions","author":"Guerra\u2010Guimar\u00e3es L","year":"2008","journal-title":"Proc. 22nd Int. Conf. on Coffee Science"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2008.04.004"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.6288","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.6288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.6288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:59:43Z","timestamp":1760864383000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.6288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,26]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1002\/jsfa.6288"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.6288","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,26]]},"assertion":[{"value":"2013-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}