{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:39:58Z","timestamp":1791207598338,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T00:00:00Z","timestamp":1374624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:bold>Simultaneous quantification of liposoluble vitamins is not a new area of interest, since these compounds co\u2010determine the nutritional quality of food and feed, a field widely explored in the human and animal diet. However, the development of appropriate methods is still a matter of concern, especially when the vitamin composition is highly complex, as is the case with feed designated for laboratory animals, representing a higher health and microbiological status<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:bold>A method combining microemulsion electrokinetic chromatography (<jats:styled-content style=\"fixed-case\">MEEKC<\/jats:styled-content>) with liquid\u2013liquid extraction was developed for the determination of four fat\u2010soluble vitamins in animal feed. A separation medium consisting of 25\u2009mmol L<jats:sup>\u22121<\/jats:sup> phosphate buffer (<jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 2.5), 2\u2010propanol, 1\u2010butanol, sodium dodecyl sulfate and octane allowed the simultaneous determination of vitamins A, D, E and K within a reasonable time of 25\u2009min. The polarity of the separation voltage was reversed in view of the strongly suppressed electro\u2010osmotic flow, and the applied voltage was set at 12 <jats:styled-content style=\"fixed-case\">kV<\/jats:styled-content>. The fat\u2010soluble vitamins were separated in the order of decreasing hydrophobicity<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:bold>It was proved that the proposed <jats:styled-content style=\"fixed-case\">MEEKC<\/jats:styled-content> method was sufficiently specific and sensitive for screening fat\u2010soluble vitamins in animal feed samples after their sterilization. \u00a9 2013 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.6290","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T10:55:04Z","timestamp":1372157704000},"page":"544-551","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Quantification of the level of fat\u2010soluble vitamins in feed based on the novel microemulsion electrokinetic chromatography (<scp>MEEKC<\/scp>) method"],"prefix":"10.1002","volume":"94","author":[{"given":"Ilona","family":"Ol\u0119dzka","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk Ul. Gen. J. Hallera 107 PL\u201080\u2010416 Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk Ul. Gen. J. Hallera 107 PL\u201080\u2010416 Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Ba\u0142uch","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk Ul. Gen. J. Hallera 107 PL\u201080\u2010416 Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"B\u0105czek","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medical University of Gda\u0144sk Ul. Gen. J. Hallera 107 PL\u201080\u2010416 Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Paradziej\u2010\u0141ukowicz","sequence":"additional","affiliation":[{"name":"Tri\u2010City Central Animal Laboratory\u2014Research and Service Centre, Medical University of Gda\u0144sk Ul. D\u0119binki 1 PL\u201080\u2010211 Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Taciak","sequence":"additional","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences Ul. Instytucka 3 PL\u201005\u2010110 Jab\u0142onna Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Pastuszewska","sequence":"additional","affiliation":[{"name":"The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences Ul. Instytucka 3 PL\u201005\u2010110 Jab\u0142onna Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00733-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/46.4.345"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jaoac\/88.6.1579"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00623-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.09.062"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700100"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01021-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.10.043"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-010-1704-9"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-022X(97)01210-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JANC.0000049720.94178.5d"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00495-0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)00130-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-010-1608-8"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00163-H"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)00026-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.04.016"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1061934810030135"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2006.05.021"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200390041"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305379"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00088-1"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(98)00217-9"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.07.024"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2004.07.047"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.12.004"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00117-5"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.04.005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)01118-9"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01283-1"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-010-1486-0"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2006.03.001"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.6290","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.6290","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.6290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:59:16Z","timestamp":1760864356000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.6290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,24]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1002\/jsfa.6290"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.6290","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,24]]},"assertion":[{"value":"2012-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}