{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T18:05:58Z","timestamp":1784225158537,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T00:00:00Z","timestamp":1376265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2014,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:bold>Specific screening methods for complex food matrices are needed that enable unambiguous and sensitive detection of bio threat agents (<jats:styled-content style=\"fixed-case\">BTAs<\/jats:styled-content>) such as <jats:italic>Bacillus anthracis<\/jats:italic> spores and microbial toxins (e.g. staphylococcal enterotoxin B (<jats:styled-content style=\"fixed-case\">SEB<\/jats:styled-content>) and clostridial botulinum neurotoxins (<jats:styled-content style=\"fixed-case\">BoNTs<\/jats:styled-content>)). The present study describes an image\u2010based 96\u2010well Meso Scale Discovery (<jats:styled-content style=\"fixed-case\">MSD<\/jats:styled-content>) electrochemiluminescence (<jats:styled-content style=\"fixed-case\">ECL<\/jats:styled-content>) assay for simultaneous detection of <jats:styled-content style=\"fixed-case\">BTAs<\/jats:styled-content> in dairy milk products<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:bold>The limit of detection of this <jats:styled-content style=\"fixed-case\">ECL<\/jats:styled-content> assay is 40 pg <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> for <jats:styled-content style=\"fixed-case\">BoNT<\/jats:styled-content>\/A complex, 10 pg <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> for <jats:styled-content style=\"fixed-case\">SEB<\/jats:styled-content> and 40\u2009000 <jats:styled-content style=\"fixed-case\">CFU mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> for <jats:italic>Bacillus anthracis<\/jats:italic> spores in dairy milk products. The <jats:styled-content style=\"fixed-case\">ECL<\/jats:styled-content> assay was successfully applied to screen type A <jats:italic>Clostridium botulinum<\/jats:italic> outbreak strains<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:bold>The results of the study indicate that this <jats:styled-content style=\"fixed-case\">ECL<\/jats:styled-content> assay is very sensitive, rapid (&lt;6 h) and multiplex in nature. The <jats:styled-content style=\"fixed-case\">ECL<\/jats:styled-content> assay has potential for use as an <jats:italic>in vitro<\/jats:italic> screening method for <jats:styled-content style=\"fixed-case\">BTAs<\/jats:styled-content> over other comparable immunoassays. \u00a9 2013 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.6310","type":"journal-article","created":{"date-parts":[[2013,7,19]],"date-time":"2013-07-19T10:12:42Z","timestamp":1374228762000},"page":"707-712","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["An electrochemiluminescence assay for the detection of bio threat agents in selected food matrices and in the screening of <i>Clostridium botulinum<\/i> outbreak strains associated with type A botulism"],"prefix":"10.1002","volume":"94","author":[{"given":"Amita","family":"Sachdeva","sequence":"first","affiliation":[{"name":"Division of Microbiology, Office of 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