{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:36:03Z","timestamp":1760920563102,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,10,10]],"date-time":"2013-10-10T00:00:00Z","timestamp":1381363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:bold>Various studies have been conducted to evaluate the effect of <jats:italic>Bt<\/jats:italic> crops on animals. Insect\u2010resistant bioassays have revealed that the chitinase\u2013<jats:styled-content style=\"fixed-case\">BmkIT<\/jats:styled-content> combination could be used as a new pest\u2010resistant gene source and might be a complementary alien gene source to the <jats:italic>Bt<\/jats:italic> toxin gene. So it is necessary to assay the effects of chitinase\u2013<jats:styled-content style=\"fixed-case\">BmkIT<\/jats:styled-content> plants on animals<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:bold>Forty 40\u2010day old New Zealand White Rabbits (<jats:italic>Oryctolagus cuniculus<\/jats:italic>) were fed for 60 consecutive days with formulated feed containing dehydrated poplar (<jats:italic>Populus cathayana<\/jats:italic> Rehd) leaves harbouring chitinase\u2013<jats:styled-content style=\"fixed-case\">BmkIT<\/jats:styled-content> gene combination or untransformed counterparts, and the potential toxicological effects of transgenic leaves on rabbits were explored. The results of the growth study revealed no significant differences for daily weight gain, feed intake and feed conversion ratio where they were 101.6%, 99.2% and 97.8% of the treatment compared to the control, respectively. No obvious pathological change was observed in the small intestine, stomach, spleen, kidney, lung, heart, bladder, pancreas, prostate and ovary. Electron microscopy observations of liver cells and renal cells showed they were both normal in the two groups. No feed\u2010derived chitinase, <jats:styled-content style=\"fixed-case\">BmkIT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NPTII<\/jats:styled-content> genes were found in small intestine, blood, or leg muscle samples although they were detected in the formulated feed<\/jats:bold>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:bold>We conclude that the processed poplar leaves with foreign chitinase\u2013<jats:styled-content style=\"fixed-case\">BmkIT<\/jats:styled-content> genes had no obviously harmful effects on rabbits. \u00a9 2013 Society of Chemical Industry<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.6388","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T15:27:03Z","timestamp":1378913223000},"page":"1163-1167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of dietary transgenic poplar leaf pellets on performance and tissues in rabbits"],"prefix":"10.1002","volume":"94","author":[{"given":"Liyan","family":"Yang","sequence":"first","affiliation":[{"name":"Department Life Science Shanxi Normal University Linfen 041004 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"The Biotechnology Research Centre Shanxi Academy of Agricultural Sciences Taiyuan 030031 P.R. China"},{"name":"Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau Ministry of Agriculture Taiyuan 030031 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixue","family":"Wang","sequence":"additional","affiliation":[{"name":"The Biotechnology Research Centre Shanxi Academy of Agricultural Sciences Taiyuan 030031 P.R. China"},{"name":"Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau Ministry of Agriculture Taiyuan 030031 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoshan","family":"Hao","sequence":"additional","affiliation":[{"name":"The Biotechnology Research Centre Shanxi Academy of Agricultural Sciences Taiyuan 030031 P.R. 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