{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T03:31:17Z","timestamp":1776742277335,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:00:00Z","timestamp":1750291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002446","name":"CUP","doi-asserted-by":"publisher","award":["B55F21007550003"],"award-info":[{"award-number":["B55F21007550003"]}],"id":[{"id":"10.13039\/501100002446","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Bioaerosols, which include airborne bacteria, are a growing concern for public health, especially near intensive farming operations that generate high concentrations of microorganisms. Intensive farms, such as those for cattle, can release potentially pathogenic bacteria into the air, posing risks to nearby communities and farmworkers. Traditional sampling methods are often limited, as they require stationary collection points and can miss key information about how these bioaerosols disperse. To address this issue, this study introduces a proof\u2010of\u2010concept drone\u2010based system designed to monitor and sample airborne bacteria near intensive farms.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The drone, equipped with a sampling device, was used to sample three different points near a livestock production farm. DNA extraction, which was also traditionally a limitation in microbial airborne molecular biology, was achieved after only 10\u2009min of flying by employing an ad hoc developed DNA extraction protocol coupled with a petroleum trap and virus DNA extraction kit. The results showed that DNA was successfully extracted, and reads were checked for quality. The primary bacterial genera found across all sites are <jats:italic>Acinetobacter<\/jats:italic> and <jats:italic>Bacillus<\/jats:italic>. While <jats:italic>Acinetobacter<\/jats:italic> includes both commensal and mastitis\u2010causing strains, <jats:italic>Bacillus<\/jats:italic> species such as <jats:italic>Bacillus subtilis<\/jats:italic> serve as probiotics in animal feed. Probiotic genera <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>Bifidobacterium<\/jats:italic> were also detected, alongside potentially pathogenic <jats:italic>Staphylococcus<\/jats:italic>, highlighting hygiene concerns. Unexpected genera, including cyanobacteria from nearby water bodies, and <jats:italic>Microvirga<\/jats:italic>, typically found in arid, metal\u2010rich soils, suggest environmental influences or contaminants.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Further development of this system can provide an early warning mechanism by tracking the presence and movement of harmful microorganisms in a 3D space surrounding the farms, allowing for safety and public health applications. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.70001","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:39:53Z","timestamp":1750379993000},"page":"7191-7198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A drone\u2010based system for sampling airborne bacteria near intensive 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