{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:37:41Z","timestamp":1791664661714,"version":"4.3.4"},"reference-count":63,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Obesity induces various co\u2010morbidities including insulin resistance.\n                      <jats:italic>Opuntia ficus\u2010indica<\/jats:italic>\n                      fruit (OFIF) is rich in various beneficial compounds with antioxidant and anti\u2010inflammatory properties. The present study evaluated the efficacy of OFIF supplementation to counteract obesity\u2010induced insulin resistance in comparison with an euglycemic agent, chromium picolinate (CrPi), in high\u2010fat\u2010diet (HFD)\u2010fed mice. Molecular investigations associated with the observed effects as well as the evaluation of OFIF impact on the composition of gut microbiota in HFD mice were also carried out. Glucose metabolic parameters, oxidative stress and inflammation\u2010related biomarkers were analysed in liver and adipose tissue of HFD, HFD\u2009+\u2009OFIF and HFD\u2009+\u2009CrPI mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      OFIF supplementation, as well CrPi, improved glucose tolerance and insulin sensitivity (evaluated as HOMA\u2010IR) and increased hepatic and adipose tissue insulin receptor expression. Moreover, similar to CrPi, OFIF markedly decreased hepatic oxidative stress and inflammation in terms of NRF\u20102, SOD\u20102, NF\u2010kB, iNOS and COX\u20102 protein expression levels. In adipose tissue, it reduced\n                      <jats:italic>IL\u20101\u03b2<\/jats:italic>\n                      ,\n                      <jats:italic>IL\u20106<\/jats:italic>\n                      ,\n                      <jats:italic>TNF\u2010\u03b1<\/jats:italic>\n                      gene expression and the density of crown\u2010like structures. In HFD mice, OFIF supplementation increased the presence of bacterial genera positively correlated to beneficial effects such as\n                      <jats:italic>Alloprevotella<\/jats:italic>\n                      ,\n                      <jats:italic>Lachnospiraceae NK4A136 group<\/jats:italic>\n                      and\n                      <jats:italic>NK4A214_group<\/jats:italic>\n                      , while it reduced the abundance of some bacterial genera associated with harmful actions (\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Romboutsia<\/jats:italic>\n                      ,\n                      <jats:italic>Sporosarcina<\/jats:italic>\n                      ,\n                      <jats:italic>Lachnoclostridium<\/jats:italic>\n                      and\n                      <jats:italic>Enterohabdus<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      Our research demonstrates for the first time the euglycemic potential of OFIF and its ability to counteract obesity\u2010induced insulin resistance through not only its antioxidative and anti\u2010inflammatory properties but also its positive modulation of gut microbiota. \u00a9 2025 The Author(s).\n                      <jats:italic>Journal of the Science of Food and Agriculture<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70038","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T06:04:37Z","timestamp":1753509877000},"page":"7868-7880","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>\n                      <i>Opuntia ficus\u2010indica<\/i>\n                    <\/scp>\n                    fruit consumption improves insulin resistance in mice with diet\u2010induced obesity"],"prefix":"10.1002","volume":"105","author":[{"given":"Simona","family":"Terzo","sequence":"first","affiliation":[{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF) University of Palermo  Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pasquale","family":"Calvi","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF) University of 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