{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T13:28:26Z","timestamp":1782221306187,"version":"3.54.5"},"reference-count":58,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS\u201041"],"award-info":[{"award-number":["CARS\u201041"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007062","name":"Yangzhou University","doi-asserted-by":"publisher","award":["SJCX23_2008"],"award-info":[{"award-number":["SJCX23_2008"]}],"id":[{"id":"10.13039\/501100007062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>The global rise in antibiotic use, particularly tetracyclines (TCs) in animal husbandry, poses significant risks to food safety and public health due to residual accumulation and bacterial resistance. Existing detection methods for TCs in dairy products often lack efficiency, sensitivity, or the capability for simultaneous multi\u2010residue analysis. Developing a rapid, precise, and cost\u2010effective method to monitor TCs in compliance with international maximum residue limits remains an urgent need.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We developed a streamlined approach integrating a three\u2010step purification process (PRiME HLB cartridges) with ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS). The method utilizes 0.1\u2009mol\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      Na\n                      <jats:sub>2<\/jats:sub>\n                      EDTA\u2013McIlvaine buffer for extraction, achieving 83.11\u2013107.61% recovery rates with &lt;\u20095.43% relative standard deviation (RSD). Optimized chromatographic separation on a bridged ethyl\u2010siloxane and silica hybrid (BEH) C\n                      <jats:sub>18<\/jats:sub>\n                      column enabled simultaneous detection of 11 TCs (including parent compounds and metabolites) within 9\u2009min. Sensitivity was exceptional, with limits of detection and limits of quantification of 0.02\u20130.83\u2009\u03bcg\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 0.07\u20132.78\u2009\u03bcg\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively. Intra\u2010\/inter\u2010day precision (RSD 3.03\u20139.33%) and compliance with European Union\/Food and Drug Administration (EU\/FDA) were validated using real milk and milk powder samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>This method combines rapid sample preparation with UPLC\u2013MS\/MS for simultaneous quantification of 11 TCs in dairy products. Its high accuracy, sensitivity, and compliance with global regulatory standards make it indispensable for large\u2010scale food safety monitoring, effectively addressing antibiotic residue risks and supporting international trade harmonization. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70088","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T10:19:28Z","timestamp":1753784368000},"page":"8525-8539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["An optimized\n                    <scp>SPE<\/scp>\n                    \u2010\n                    <scp>UPLC<\/scp>\n                    \u2013\n                    <scp>MS<\/scp>\n                    \/\n                    <scp>MS<\/scp>\n                    method for simultaneous quantification of 11 tetracyclines in dairy products"],"prefix":"10.1002","volume":"105","author":[{"given":"Pengfei","family":"Gao","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University  Yangzhou China"},{"name":"Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boxing","family":"Yin","sequence":"additional","affiliation":[{"name":"Experimental Farm of Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renqin","family":"Yang","sequence":"additional","affiliation":[{"name":"Experimental Farm of Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sihui","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genxi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2788-4323","authenticated-orcid":false,"given":"Kaizhou","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University  Yangzhou China"},{"name":"College of Animal Science and Technology, Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.16.020916"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19127246"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2024.114491"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1315497"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-10266-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13229"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.02.010"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci7020048"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3329\/bjvm.v2i2.2538"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10050578"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.02.019"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00271-06"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2021.01.075"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx039"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.01.033"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4465"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1986.9694057"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl224"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2009.01138.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2021.110450"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.115539"},{"key":"e_1_2_9_23_1","unstructured":"Codex alimentarius commission Maximum residue limits (MRLs) and risk management recommendations (RMRs) for residues of veterinary drugs in foods(2018)."},{"key":"e_1_2_9_24_1","volume-title":"Commission Regulation (EU) NO. 37\/2010 of 22 December 2009 On Pharmacologically Active Substances and their Classification Regarding Maximum Residue Limits in Foodstuffs of Animal Origin","author":"The European Medicines Agency","year":"2010"},{"key":"e_1_2_9_25_1","volume-title":"National Food Safety Standard\u2010Maximum Residue Limits of Veterinary Drugs in Foods","author":"Ministry of Agriculture and Rural Affairs of the People's Republic of China, National Health Commission of the People's Republic of China, State Administration of Market Regulation","year":"2019"},{"key":"e_1_2_9_26_1","unstructured":"U.S. Food and Drug Administration CFR\u2010Code of federal regulations title 21 part 556 tolerances for residue of new animal drugs in food(2019)."},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.01.058"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-019-4012-6"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.04.021"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.11.008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.08.004"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.10.012"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b00748"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-022-04442-4"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-020-02688-4"},{"key":"e_1_2_9_36_1","unstructured":"The European Communities Commission implementing regulation (EU) 2021\/808 of 22 march 2021 on the performance of analytical methods for residues of pharmacologically active substances used in food\u2010producing animals and on the interpretation of results as well as on the methods to be used for sampling and repealing decisions 2002\/657\/EC and 98\/179\/EC. Off J Eur Union 180:84\u2013114(2021)."},{"key":"e_1_2_9_37_1","volume-title":"Guidance for Industry: Bioanalytical Method Validation","author":"U.S. Department of Health and Human Services, Food and Drug Administration","year":"2018"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c00296"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2004.03.008"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2015.08.012"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx168"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2021.117196"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios12090717"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.133170"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.132697"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.07.025"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2024.106317"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.137049"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.135861"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2017.1397773"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2021.104265"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2020.105437"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.09.005"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/d1ay00154j"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations6020021"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2016.04.050"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.06.064"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7ra08203g"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701514"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.70088","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T08:02:22Z","timestamp":1762588942000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.70088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":58,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/jsfa.70088"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.70088","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,29]]},"assertion":[{"value":"2025-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}