{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T15:58:23Z","timestamp":1777564703759,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32325040"],"award-info":[{"award-number":["32325040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Male reproductive health is intricately linked to dietary components and gut microbiota balance. Osteopontin (OPN) deficiency and gut dysbiosis are associated with impaired spermatogenesis, yet the synergistic effects of probiotics and OPN remain unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In OPN\u2010deficient mice, combined intervention with\n                      <jats:italic>Bifidobacterium longum<\/jats:italic>\n                      subsp.\n                      <jats:italic>infantis<\/jats:italic>\n                      B8762 and OPN increased gut microbiota alpha diversity and upregulated key reproductive genes (\n                      <jats:italic>Etv4<\/jats:italic>\n                      ,\n                      <jats:italic>Adamts16<\/jats:italic>\n                      ,\n                      <jats:italic>Prok2<\/jats:italic>\n                      ,\n                      <jats:italic>Gpr55<\/jats:italic>\n                      ,\n                      <jats:italic>Rad54b<\/jats:italic>\n                      ). Testicular histology revealed restored spermatogenic cell density and seminiferous tubule integrity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This study demonstrates that co\u2010administration of B8762 and OPN alleviates reproductive dysfunction via gut microbiota modulation and testicular gene regulation, offering a novel nutritional strategy for male fertility enhancement. \u00a9 2025 The Author(s).\n                      <jats:italic>Journal of the Science of Food and Agriculture<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70121","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T03:51:01Z","timestamp":1755834661000},"page":"8827-8835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Synergistic effects of\n                    <scp>\n                      <i>Bifidobacterium longum<\/i>\n                    <\/scp>\n                    subsp.\n                    <scp>\n                      <i>infantis<\/i>\n                      B8762\n                    <\/scp>\n                    and milk\u2010derived osteopontin on male reproductive health in mice"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2646-5265","authenticated-orcid":false,"given":"Chunyan","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangqi","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zerong","family":"Lu","sequence":"additional","affiliation":[{"name":"Health and Happiness (H&amp;H) Group, H&amp;H Research, China Research and Innovation Center  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Fourth Medical Center of PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7605-2048","authenticated-orcid":false,"given":"Zhihong","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University; 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