{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T09:05:38Z","timestamp":1783933538067,"version":"3.55.0"},"reference-count":67,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Saffron (\n                      <jats:italic>Crocus sativus<\/jats:italic>\n                      L.), a Chinese herbal medicine of significant medicinal value, is extensively cultivated worldwide. However, fungal pathogen\u2010induced corm rot disease poses a major challenge to saffron cultivation, causing substantial economic losses and long\u2010standing concern among researchers and industry professionals. The presemt study aimed to accurately and rapidly identify\n                      <jats:italic>Fusarium oxysporum<\/jats:italic>\n                      f. sp. crocus (FOSC) as a crocus\u2010specific pathogen using high\u2010throughput sequencing technology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      High\u2010throughput sequencing successfully confirmed FOSC as the pathogen responsible for crocus corm rot, with subsequent tolerance gene predictions indicating its resistance to multiple antibiotics. Among eight tested plant essential oils,\n                      <jats:italic>Cinnamomum cassia<\/jats:italic>\n                      essential oil (CC\u2010EO) exhibited the strongest antifungal activity against FOSC. Cellular\u2010level investigations revealed that CC\u2010EO combats FOSC by compromising spore cell membrane integrity, inducing nuclear DNA fragmentation and promoting reactive oxygen species accumulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This study represents the first application of high\u2010throughput sequencing to identify the crocus\u2010specific FOSC, and demonstrates that CC\u2010EO holds significant potential for preventing and controlling saffron corm rot through its multi\u2010target antifungal mechanism. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  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