{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:53:19Z","timestamp":1790139199938,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Breast cancer (BC) is a life\u2010threatening disease, with approximately 60% of cases progressing to breast cancer lung metastasis (BCLM). Chamuangone, a key bioactive constituent derived from the leaves of the Thai plant\n                      <jats:italic>Garcinia cowa<\/jats:italic>\n                      Roxb., has demonstrated significant potential as an anticancer agent. To evaluate its anticancer effects, chamuangone was purified from the leaves and assessed through\n                      <jats:italic>in silico<\/jats:italic>\n                      ,\n                      <jats:italic>in vitro<\/jats:italic>\n                      , and\n                      <jats:italic>in vivo<\/jats:italic>\n                      studies using a mouse model of BC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The results revealed that chamuangone (10\u2009mg\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) inhibited cancer cell proliferation by inducing G0\/G1 phase arrest and apoptosis in 4T1\u2010luciferase (4T1\u2010luc) and Michigan Cancer Foundation\u20107 (MCF\u20107) cells. It also modulated the levels of key apoptotic markers, including B\u2010cell lymphoma 2 (Bcl\u20102), Bcl\u20102\u2010associated X protein (Bax), and caspase\u20103. Similarly,\n                      <jats:italic>in vivo<\/jats:italic>\n                      studies demonstrated that chamuangone significantly (\n                      <jats:italic>P\u2009&lt;<\/jats:italic>\n                      \u20090.05) inhibited breast tumor growth and lung metastasis at a dose of 100\u2009mg\u2009kg\u207b\u00b9 body weight, in comparison with the standard drug doxorubicin (5\u2009mg\u2009kg\u207b\u00b9 body weight) in mice. Immunohistochemical analysis further revealed that chamuangone modulated several BCLM\u2010associated markers, including estrogen receptor (ER), progesterone receptor (PR), Ki\u201067 antigen (Ki\u201067), cluster of differentiation 44 (CD44), E\u2010cadherin, N\u2010cadherin, vascular endothelial growth factor A (VEGFA), interleukin\u20106 (IL\u20106), and tumor necrosis factor\u2010\n                      <jats:italic>\u03b1<\/jats:italic>\n                      (TNF\u2010\n                      <jats:italic>\u03b1<\/jats:italic>\n                      ), in both breast tumors and lung tissues. These effects contributed to the inhibition of inflammation, angiogenesis, epithelial\u2013mesenchymal transition (EMT), cancer cell proliferation, and metastasis.\n                      <jats:italic>In silico<\/jats:italic>\n                      studies also demonstrated strong interactions between chamuangone and BCLM\u2010related targets, along with encouraging pharmacokinetic and toxicokinetic profiles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This investigation highlights the potential uses of chamuangone as a chemopreventive agent and functional component for managing the advanced stages of BC. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70175","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T06:50:06Z","timestamp":1758610206000},"page":"469-481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chamuangone purified from\n                    <i>Garcinia cowa<\/i>\n                    inhibits inflammation, angiogenesis, epithelial\u2013mesenchymal transition, and metastasis in breast cancer"],"prefix":"10.1002","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6862-0554","authenticated-orcid":false,"given":"Asad Ur","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany Faculty of Pharmaceutical Sciences, Prince of Songkla University  Hat\u2010Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveed Ullah","family":"Khan","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Basit","sequence":"additional","affiliation":[{"name":"College of Pharmacy Shenzhen Technology University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany Faculty of Pharmaceutical Sciences, Prince of Songkla University  Hat\u2010Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Jiangnan University  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1097-8822","authenticated-orcid":false,"given":"Pharkphoom","family":"Panichayupakaranant","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany Faculty of Pharmaceutical Sciences, Prince of Songkla University  Hat\u2010Yai Thailand"},{"name":"Phytomedicine and Pharmaceutical Biotechnology Excellence Centre Faculty of Pharmaceutical Sciences, Prince of Songkla University  Hat\u2010Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2404625"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21863"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2022.02.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2024.104525"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.07.088"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118625"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.6000\/1929-2279.2017.06.02.3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.02.020"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2011.209"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2016.11.034"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2017.05.049"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2022.07.002"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddtec.2004.11.007"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/3613268"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1060464"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-86809-y"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr2889"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.02.008"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-65821-8"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115748928251754230922095544"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-017-0072-x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-021-06722-1"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113932"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.15537\/smj.2023.44.3.20220768"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-024-00714-6"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-74608-w"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2007.02897.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174455"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3410"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051325"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2014_026"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.866014"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605530"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-006-9005-3"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24044002"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr3367"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800911795655985"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2023.154923"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.70175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T03:45:33Z","timestamp":1763437533000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.70175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,22]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,15]]}},"alternative-id":["10.1002\/jsfa.70175"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.70175","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,22]]},"assertion":[{"value":"2025-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}