{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T20:10:25Z","timestamp":1774383025706,"version":"3.50.1"},"reference-count":83,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100019683","name":"College of Agriculture, Ferdowsi University of Mashhad","doi-asserted-by":"publisher","award":["3\/55090"],"award-info":[{"award-number":["3\/55090"]}],"id":[{"id":"10.13039\/501100019683","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gut microbiota dysbiosis is associated with diarrhea\u2010predominant irritable bowel syndrome (IBS\u2010D). Visceral hypersensitivity (VH) is a hallmark symptom.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010five female rats were allocated to either a control group (sham stress,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095) or a stressed group exposed to a 10\u2010day water avoidance (WA) stress protocol to induce VH (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920). After stress induction, stressed rats received a 4 week intervention with phosphate\u2010buffered saline (PBS),\n                      <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                      subsp.\n                      <jats:italic>lactis<\/jats:italic>\n                      BB\u201012 (P1),\n                      <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                      ATCC 14917 (P2), or their combination (P1P2). Control rats were administered PBS. Visceral hypersensitivity was assessed using abdominal withdrawal reflex (AWR) scores, and metabolic changes in plasma, liver, and distal colon were analyzed using proton nuclear magnetic resonance (\n                      <jats:sup>1<\/jats:sup>\n                      H NMR)\u2010based metabolomics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>\n                      Water avoidance stress induced VH, as evidenced by higher AWR scores (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) and lower pain thresholds compared to controls (38.47\u2009\u00b1\u20093.78 versus 29.48\u2009\u00b1\u20092.52;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001). Probiotics significantly reduced AWR scores at 20 and 80\u2009mmHg and increased pain thresholds in comparison with the WA\u2009+\u2009PBS group (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). Metabolomic profiling revealed that WA\u2009+\u2009PBS rats showed significant dysregulation in energy, amino acid, one\u2010carbon, and lipid metabolism. Notable changes included elevated sarcosine, acetate, taurine, glutamate, tryptophan, 2\u2010hydroxybutyrate, urea, and allantoin, with reduced\n                      <jats:italic>O<\/jats:italic>\n                      \u2010acetylcarnitine in plasma; increased succinate,\n                      <jats:italic>myo<\/jats:italic>\n                      \u2010inositol, isoleucine, threonine, glutamine, betaine, pyroglutamate, and\n                      <jats:italic>O<\/jats:italic>\n                      \u2010phosphocholine with decreased taurine in the liver; and various amino acids, ketone bodies, glucose, glycerol, and one\u2010carbon metabolites in the colon (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001). Probiotic supplementation largely restored these metabolic changes, with clustering analyses supporting the normalization of metabolic signatures.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Probiotic supplementation ameliorated VH and reversed IBS\u2010D\u2010associated metabolic disruptions, illustrating its therapeutic potential and providing insights into the underlying mechanisms. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70190","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T13:55:32Z","timestamp":1758894932000},"page":"620-631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of probiotic administration of\n                    <i>Bifidobacterium animalis<\/i>\n      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Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4771-5359","authenticated-orcid":false,"given":"Masoud","family":"Yavarmanesh","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Agriculture Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam M.","family":"Matin","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Ferdowsi University of Mashhad  Mashhad Iran"},{"name":"Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid","family":"Noorbakhsh","sequence":"additional","affiliation":[{"name":"Department of Engineering, Shahreza Branch Islamic Azad University  Isfahan 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