{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T09:45:48Z","timestamp":1785836748175,"version":"3.56.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Gynura procumbens<\/jats:italic>\n                      is a medicinal plant widely recognized for its antioxidant and anti\u2010inflammatory properties. Despite its traditional use in Southeast Asia, its potential role in promoting muscle health has not been thoroughly investigated. Sarcopenia, characterized by the progressive loss of muscle mass and strength during aging, remains a significant public health concern with limited therapeutic options. This study aimed to enhance the phytochemical content of\n                      <jats:italic>G. procumbens<\/jats:italic>\n                      leaves through ultrasound treatment and to evaluate the biological effects of treated extracts on muscle atrophy in a C2C12 myoblast and myotube model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Ultrasound treatment for 10\u2009min significantly increased the activities of catalase, peroxidase, phenylalanine ammonia\u2010lyase, and tyrosine ammonia\u2010lyase. These enzymatic changes were associated with elevated levels of phenolic acids and flavonoids, including quercetin, rutin, and kaempferol. The 10\u2009min ultrasound\u2010treated\n                      <jats:italic>G. procumbens<\/jats:italic>\n                      (UTGP) extract enhanced cell viability under oxidative stress conditions and promoted the expression of myogenic differentiation markers in C2C12 cells. Targeted and untargeted metabolomics analyses confirmed the accumulation of key bioactive compounds and their strong correlation with muscle\u2010protective activities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      Ultrasound treatment is an effective postharvest strategy to enhance the phytochemical profile and functional properties of\n                      <jats:italic>G. procumbens<\/jats:italic>\n                      . The 10\u2009min UTGP extract demonstrated significant potential for preventing muscle atrophy by improving cellular antioxidant capacity and promoting muscle differentiation. These findings provide a scientific basis for the development of\n                      <jats:italic>G. procumbens<\/jats:italic>\n                      \u2010based functional foods or nutraceuticals targeting age\u2010related muscle loss. \u00a9 2025 The Author(s).\n                      <jats:italic>Journal of the Science of Food and Agriculture<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70335","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T07:15:04Z","timestamp":1763709304000},"page":"2270-2279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of post\u2010harvest ultrasound treatment on phytochemical enhancement in\n                    <i>Gynura procumbens<\/i>\n                    leaves and their protection against oxidative stress\u2010induced muscle atrophy"],"prefix":"10.1002","volume":"106","author":[{"given":"Taemin","family":"Jeong","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology Chungbuk National University  Cheongju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonghwa","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology Chungbuk National University  Cheongju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeehye","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology Gyeongkuk National University  Andong Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0349-4483","authenticated-orcid":false,"given":"Heon\u2010Sang","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology Chungbuk National University  Cheongju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4059-4195","authenticated-orcid":false,"given":"Hana","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology Chungbuk National University  Cheongju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5557-4229","authenticated-orcid":false,"given":"Junsoo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology Chungbuk National University  Cheongju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-food-030212-182537"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.05.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2023.106353"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2011.04.002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27165256"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2015.01.011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2023.106345"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLR.0000000000000166"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2015.12.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11040755"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20140317"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2015.05.019"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.504624"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15129008"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e31828685b3"},{"key":"e_1_2_8_17_1","first-page":"99","article-title":"Antiproliferative effect of Gynura procumbens (Lour.) 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