{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:13:35Z","timestamp":1791526415569,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["MOST 109\u20102410\u2010H029\u2010034\u2010MY3"],"award-info":[{"award-number":["MOST 109\u20102410\u2010H029\u2010034\u2010MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Skeletal muscle atrophy occurs when muscle mass and strength decrease due to aging, starvation, cancer, and cachectic diseases. Recently, an increasing focus has been on investigating potato protein's nutritional and functional properties and its wide\u2010ranging applications. Steroids, especially glucocorticoids, have been extensively examined and found to cause muscle atrophy. Recently, we found that PP902 prevents hyperglycemia\u2010induced muscle atrophy in cultured myotubes, although its impact on steroid\u2010induced muscle atrophy remains unclear. Therefore, this study aimed to investigate if the potato peptide PP902 could prevent steroid\u2010induced muscle atrophy\n                      <jats:italic>in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The MTT assay showed no cytotoxicity in C2C12 myotubes at a dosage of 200\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      . To study the anti\u2010atrophic effects of PP902 on muscle cell atrophy, C2C12 myotubes were treated with 100\u2009\u03bcmol\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      dexamethasone (DEX) and\/or 100\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      PP902 for 24\u2009h. DEX\u2010treated cells showed reduced myotube diameter compared to control cells, while PP902 administration prevented muscular atrophy. Next, western blot analysis and reverse transcription quantitative polymerase chain reaction were used to measure MAFbx and MuRF1 protein and mRNA levels, respectively. Results showed that PP902 dramatically decreased MAFbx and MuRF1 expression. Additionally, PP902 treatment in C2C12 cells produced a dose\u2010dependent increase in myosin heavy chain (MHC) and a decrease in phosphorylated myosin heavy chain (p\u2010MHC), which is a key player in skeletal myogenesis. Furthermore, DEX treatment significantly increased the expression of glucocorticoid receptor alpha (GR\u03b1), whereas low\u2010dose PP902 (12.5, 15, and 50\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) failed to prevent GR\u03b1 activation. PP902 (100\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) suppresses GR\u03b1 activity only at higher concentrations. Additional investigations revealed that PP902 supplementation reduced DEX\u2010induced FOXO1 activity and enhanced p\u2010FoxO1 and Akt activation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Taken together, this study indicates that PP902 inhibits MAFbx and MuRF1 expression associated with inhibiting FoxO1 in C2C12 cells, potentially useful for developing anti\u2010atrophy functional foods. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70371","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T06:25:52Z","timestamp":1765002352000},"page":"2388-2396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Potato protein hydrolysate (\n                    <scp>PP902<\/scp>\n                    ) inhibits dexamethasone\u2010induced muscle atrophy by regulating\n                    <scp>MuRF1<\/scp>\n                    and\n                    <scp>MAFbx<\/scp>\n                    expression in\n                    <scp>C2C12<\/scp>\n                    myotubes\n                    <scp>\n                      <i>in vitro<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"106","author":[{"given":"KJ Senthil","family":"Kumar","sequence":"first","affiliation":[{"name":"Center for General Education, National Chung Hsing University  Taichung Taiwan"},{"name":"Department of Biotechnology Saveetha Institute of Medical and Technical Sciences, Saveetha University  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ju","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Surgery Taichung Veterans General Hospital  Taichung Taiwan"},{"name":"Department of Animal Science and Biotechnology College of Agriculture 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