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Egg yolk phosphopeptides (EYPPs) possess excellent metal\u2010chelating potential due to their abundant functional groups, making them ideal carriers for iron fortification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      This study synthesized and characterized an egg yolk phosphopeptide\u2010ferrous (EYPP\u2010Fe) chelate, optimizing reaction conditions as follows: 7.5% peptide concentration, 3:1 peptide\u2010to\u2010iron salt mass ratio, 55\u2009\u00b0C reaction temperature, pH\u20097, and 40\u2009min reaction time. Structural analyses via ultraviolet\u2013visible, fluorescence, X\u2010ray diffraction, thermogravimetric analysis, and Fourier transform infrared spectroscopy confirmed chelate formation, with ferrous ions coordinating with EYPP's amino, carboxyl, and phosphate groups.\n                      <jats:italic>In vitro<\/jats:italic>\n                      assays demonstrated EYPP\u2010Fe's robust stability against heat, salt, acid, and gastrointestinal digestion. In a zebrafish model of phenylhydrazine\u2010induced anemia, EYPP\u2010Fe significantly ameliorated anemia (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), increasing cardiac erythrocyte staining intensity by 78.57% compared to the model group \u2013 outperforming ferrous pyrophosphate.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>These findings validate EYPP\u2010Fe as a promising iron supplement candidate with enhanced stability and bioavailability, offering a viable solution for IDA mitigation. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  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