{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T18:03:17Z","timestamp":1779818597784,"version":"3.53.1"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFA1011900"],"award-info":[{"award-number":["2024YFA1011900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFA1011903"],"award-info":[{"award-number":["2024YFA1011903"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Sea buckthorn (\n                      <jats:italic>Hippophae rhamnoides<\/jats:italic>\n                      ) is a medicinal plant recognized for its therapeutic value. Although its polysaccharides are known to be bioactive, their precise structural characteristics and direct anti\u2010inflammatory mechanisms remain largely undefined. This study isolated and purified two novel homogeneous polysaccharides, SBP50\u20100 and SBP70\u20100, from sea buckthorn aiming to elucidate their detailed structures and evaluate their anti\u2010inflammatory activities\n                      <jats:italic>in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      SBP50\u20100 and SBP70\u20100 were identified as neutral polysaccharides with molecular weights of 1.04\u2009\u00d7\u200910\n                      <jats:sup>4<\/jats:sup>\n                      \u2009Da and 6.45\u2009\u00d7\u200910\n                      <jats:sup>3<\/jats:sup>\n                      \u2009Da, respectively. Monosaccharide analysis indicated they are composed of mannose, galactose, glucose, rhamnose and arabinose. Structural characterization via methylation and NMR spectroscopy revealed that both compounds possess unique, highly branched backbones, distinct from the linear chains often reported in literature. In lipopolysaccharide\u2010stimulated RAW264.7 macrophages, treatment with SBP50\u20100 and SBP70\u20100 significantly inhibited nitric oxide production. Furthermore, they effectively suppressed the protein expression of key pro\u2010inflammatory mediators, including interleukin\u20106, tumor necrosis factor\u2010\n                      <jats:italic>\u03b1<\/jats:italic>\n                      and inducible nitric oxide synthase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>These findings demonstrate that SBP50\u20100 and SBP70\u20100 possess potent anti\u2010inflammatory properties. This bioactivity is likely attributed to their specific structural architectures, particularly the highly branched arabinogalactan side chains and mannose\u2010rich motifs that facilitate interaction with macrophage receptors. Consequently, these polysaccharides show significant promise as potential candidates for the development of natural anti\u2010inflammatory functional foods or pharmaceutical agents. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70445","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:25:30Z","timestamp":1769689530000},"page":"3526-3539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural characterization and anti\u2010inflammatory activity analysis of novel polysaccharides extracted from sea buckthorn"],"prefix":"10.1002","volume":"106","author":[{"given":"Xiaoge","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Component\u2010based Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin 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