{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T17:11:21Z","timestamp":1778346681480,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360952"],"award-info":[{"award-number":["82360952"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Premature ovarian insufficiency (POI) often leads to infertility and long\u2010term health complications. Processed\n                      <jats:italic>Polygonum multiflorum<\/jats:italic>\n                      Thunb. (PM) is a traditional medicinal and edible plant widely used in health supplements and herbal medicine. It has shown potential in mitigating ovarian aging but its mechanisms against POI remain unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      This study integrated network pharmacology, molecular docking, and experimental validation to explore the therapeutic mechanism of PM in POI. Bioactive compounds in PM were identified by liquid chromatography\u2013mass spectrometry and literature screening, yielding 91 compounds targeting 320 POI\u2010related genes. Core targets included\n                      <jats:italic>AKT1, PIK3CA,<\/jats:italic>\n                      and\n                      <jats:italic>PIK3R1<\/jats:italic>\n                      , with pathway enrichment highlighting the PI3K\/AKT signaling pathway, which was further supported by molecular docking. In a rat model of POI, treatment with either low\u2010 or high\u2010dose PM restored estrous cyclicity, improved serum follicle\u2010stimulating hormone (FSH) and estradiol (E\n                      <jats:sub>2<\/jats:sub>\n                      ) levels, improved follicular development, and increased the expression of bone morphogenetic protein 15 (BMP\u201015), growth differentiation factor 9 (GDF\u20109), and key PI3K\/AKT proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Polygonum multiflorum<\/jats:italic>\n                      Thunb. may alleviate POI by activating the PI3K\/AKT pathway, restoring hormonal balance, reducing follicular atresia, and preserving ovarian reserve. These findings underscore the potential of PM as a natural therapeutic resource for POI management. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70545","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T05:01:22Z","timestamp":1773205282000},"page":"4666-4677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic mechanism of\n                    <i>Polygonum multiflorum<\/i>\n                    Thunb. in premature ovarian insufficiency: an integration of network pharmacology, molecular docking, and experimental validation"],"prefix":"10.1002","volume":"106","author":[{"given":"Jinhong","family":"Li","sequence":"first","affiliation":[{"name":"College of Basic Chinese Medicine Guizhou University of Traditional Chinese Medicine  Guiyang 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