{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:04:52Z","timestamp":1788998692475,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Dipeptidyl peptidase IV (DPP\u2010IV) inhibitors are well known medication for type 2 diabetes mellitus. The present study aimed to prepare and screen DPP\u2010IV inhibitory peptides from phycocyanin (PC) and investigate their inhibition types and cellular transportation mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      PC was hydrolyzed using four different enzymes, Neutrase hydrolysates (10.0\u2009mg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) showed the best DPP\u2010IV inhibitory activity, with an percentage inhibitory rate of 73.65%. The optimal hydrolysis conditions obtained by single\u2010factor experiments were: neutrase, enzyme concentration of 3.0%, solid\u2013liquid ratio of 1:30\u2009g\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , hydrolysis time of 3.0\u2009h, hydrolysis temperature of 50.0\u2009\u00b0C and pH\u20097.0, under which the hydrolysates showed an IC\n                      <jats:sub>50<\/jats:sub>\n                      value of 2.72\u2009\u00b1\u20090.15\u2009mg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      . Gastrointestinal digestion can improve the DPP\u2010IV inhibition of hydrolyates. Animal experiments demonstrated that oral administration of PC hydrolysates significantly reduced the FBG and glycated serum protein levels, improved glucose tolerance ability, attenuated insulin resistance, and alleviated lipid disturbance of type II diabetic mice. Two novel DPP\u2010IV inhibitory peptides, IASY and IAGIDE, with IC\n                      <jats:sub>50<\/jats:sub>\n                      values of 0.22\u2009\u00b1\u20090.01\u2009mg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 0.38\u2009\u00b1\u20090.02\u2009mg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively, were separated and characterized from the &lt;\u20093.0\u2009kDa fraction of PC hydrolysates using affinity ultrafiltration and molecular docking techniques. Both peptides exhibited a mixed inhibition pattern against DPP\u2010IV, and bound to the active site of DPP\u2010IV mainly through hydrogen bonds and hydrophobic interactions. IASY showed better biocompatibility and absorption transport rate than IAGIDE in Caco\u20102 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This study showed that peptides from PC, especially IASY and IAGIDE, have therapeutic potential in blood glucose management. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70677","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T03:58:04Z","timestamp":1777262284000},"page":"6442-6454","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multidimensional study of\n                    <scp>DPP<\/scp>\n                    \u2010\n                    <scp>IV<\/scp>\n                    inhibitory peptides from phycocyanin: Screening, identification, inhibitory mechanism, hypoglycemic activity\n                    <i>in vivo<\/i>\n                    and cellular transportation"],"prefix":"10.1002","volume":"106","author":[{"given":"Lu","family":"Zhang","sequence":"first","affiliation":[{"name":"National R&amp;D Center for Freshwater Fish Processing, College of Life Science Jiangxi Normal University  Nanchang China"},{"name":"Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010jie","family":"Guo","sequence":"additional","affiliation":[{"name":"National R&amp;D Center for Freshwater Fish Processing, College of Life Science Jiangxi Normal University  Nanchang China"},{"name":"Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine  Qingdao China"},{"name":"Yantai Institute of Coastal Zone Research Chinese Academy of Sciences  Yantai 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