{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:14:00Z","timestamp":1783455240098,"version":"3.55.0"},"reference-count":60,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1300300"],"award-info":[{"award-number":["2021YFD1300300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Moringa oleifera<\/jats:italic>\n                      leaf extract (MOLE) is a carbohydrate\u2010rich plant extract that may support intestinal homeostasis. The colon, as the principal site of the fermentation of indigestible carbohydrate, is susceptible to barrier disruption. However, the effects of MOLE on colonic health in piglets and the underlying mechanisms remain unclear. This study systematically characterized the composition and\n                      <jats:italic>in vitro<\/jats:italic>\n                      antioxidant activity of MOLE, evaluated its effects on colonic health in piglets, and explored the potential mechanisms using the fermentation of colonic digesta and an intestinal porcine epithelial cell line J2 (IPEC\u2010J2) oxidative stress model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      MOLE was predominantly composed of carbohydrates and showed measurable chemical antioxidant activity. In weaned piglets, dietary MOLE alleviated lipopolysaccharide (LPS)\u2010induced colonic histological injury by suppressing\n                      <jats:italic>CD14<\/jats:italic>\n                      \u2010dependent\n                      <jats:italic>TLR4<\/jats:italic>\n                      signaling, enhancing\n                      <jats:italic>NFE2L2<\/jats:italic>\n                      \u2010\n                      <jats:italic>GCLC<\/jats:italic>\n                      \u2010mediated antioxidant defenses, up\u2010regulating\n                      <jats:italic>OCLN<\/jats:italic>\n                      and\n                      <jats:italic>MUC2<\/jats:italic>\n                      , and modulating microbial and short\u2010chain fatty acid (SCFA) profiles. In colonic digesta fermentation, MOLE supported carbohydrate\u2010driven fermentation, as indicated by rapid bacterial growth, sustained acetate\u2010dominant SCFA production, decreased pH, increased gas production, and the absence of branched\u2010chain fatty acids. In IPEC\u2010J2 cells, MOLE enhanced\n                      <jats:italic>GCLC<\/jats:italic>\n                      and\n                      <jats:italic>GPX<\/jats:italic>\n                      expression and promoted GSH\u2010\n                      <jats:italic>GPX<\/jats:italic>\n                      \u2010based antioxidant defenses under hydrogen peroxide challenge.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>MOLE alleviates inflammation and oxidative stress, thereby helping to maintain intestinal homeostasis. Together, these results support MOLE's development as a practical feed additive. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70713","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T04:10:41Z","timestamp":1778472641000},"page":"6755-6765","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Moringa oleifera<\/i>\n                    leaf extract alleviates\n                    <scp>LPS<\/scp>\n                    \u2010induced colonic injury in piglets by modulating oxidative status and microbial fermentation"],"prefix":"10.1002","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2479-360X","authenticated-orcid":false,"given":"Ziwen","family":"Liu","sequence":"first","affiliation":[{"name":"National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal 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