{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:14:20Z","timestamp":1783455260517,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2026,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>The present study aimed to valorize broccoli by\u2010products (leaves and stems) through the extraction of bioactive compounds using supercritical fluid extraction (SFE). The influence of broccoli variety, plant part and maturation stage on total phenolic content (TPC), antioxidant activity and antimicrobial properties was evaluated. Additionally, the bioactive compounds present in the extracts were identified by HPLC\u2010UV\u2010electrospray ionization\u2010tandem mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Although extraction yields were lower than those obtained with conventional methods, SFE extracts exhibited high antioxidant activity, reaching values up to 1311.8\u2009mg Trolox kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      of dry extract in the CLX 3570 variety using the DPPH assay. A strong correlation between phenolic content and antioxidant activity was observed. The extracts also showed notable antimicrobial activity, with inhibition values up to 98.09% against\n                      <jats:italic>Bacillus cereus<\/jats:italic>\n                      , particularly in the Shard cultivar. This effect was associated with the presence of specific fatty acids, such as linoleic and palmitic acids, which were more abundant in stems. Furthermore, increasing maturation stage significantly enhanced extraction yield, TPC, antioxidant activity and antimicrobial capacity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Broccoli by\u2010products represent a promising source of functional bioactive compounds. SFE emerges as a sustainable extraction technique capable of producing high\u2010quality extracts with significant biological activity, contributing to waste valorization and the development of functional ingredients for the food industry. \u00a9 2026 The Author(s).\n                      <jats:italic>Journal of the Science of Food and Agriculture<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.70718","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T06:59:42Z","timestamp":1778741982000},"page":"6789-6799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of functional metabolites in broccoli by\u2010product using green extraction with supercritical fluids"],"prefix":"10.1002","volume":"106","author":[{"given":"Iris","family":"Gudi\u00f1o","sequence":"first","affiliation":[{"name":"Nutrici\u00f3n y Bromatolog\u00eda Instituto Universitario de Investigaci\u00f3n en Recursos Agrarios (INURA), Escuela de Ingenier\u00edas Agrarias, Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3653-0762","authenticated-orcid":false,"given":"Roc\u00edo","family":"Casquete","sequence":"additional","affiliation":[{"name":"Nutrici\u00f3n y Bromatolog\u00eda Instituto Universitario de Investigaci\u00f3n en Recursos Agrarios (INURA), Escuela de Ingenier\u00edas Agrarias, Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8432-8035","authenticated-orcid":false,"given":"Mar\u00eda Jos\u00e9","family":"Benito","sequence":"additional","affiliation":[{"name":"Nutrici\u00f3n y Bromatolog\u00eda Instituto Universitario de Investigaci\u00f3n en Recursos Agrarios (INURA), Escuela de Ingenier\u00edas Agrarias, Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda de Gu\u00eda","family":"C\u00f3rdoba","sequence":"additional","affiliation":[{"name":"Nutrici\u00f3n y Bromatolog\u00eda Instituto Universitario de Investigaci\u00f3n en Recursos Agrarios (INURA), Escuela de Ingenier\u00edas Agrarias, Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7148-0596","authenticated-orcid":false,"given":"Mar\u00eda Henar","family":"Prieto","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Agrarias. Finca La Orden\u2010Valdesequera. CICYTEX (Junta de Extremadura)  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6104-8102","authenticated-orcid":false,"given":"Alberto","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Nutrici\u00f3n y Bromatolog\u00eda Instituto Universitario de Investigaci\u00f3n en Recursos Agrarios (INURA), Escuela de Ingenier\u00edas Agrarias, Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0718-58392019000100036"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13414"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13629"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2021.12.015"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2023.2195493"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.04.066"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.11.021"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23040900"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ifset.2022.103075"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.109055"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13050082"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2022.111284"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-023-04362-2"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-016-0399-4"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9121195"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.06.098"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-015-9434-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfpe.12670"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.121713"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-019-04123-8"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2021.111305"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12122417"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.07.051"},{"key":"e_1_2_7_25_1","volume-title":"En: XXVIII Congreso de la Sociedad Espa\u00f1ola de Microbiolog\u00eda; junio","author":"Hidalgo C","year":"2021"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/00032710903276646"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1565(199807\/08)9:4<196::AID-PCA395>3.0.CO;2-W"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf901121v"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9010007"},{"key":"e_1_2_7_30_1","first-page":"408","article-title":"Antimicrobial and antioxidant activities in different parts of broccoli","volume":"32","author":"Park MY","year":"2014","journal-title":"Hortic Sci Technol"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.138517"},{"key":"e_1_2_7_32_1","first-page":"1","article-title":"Anti\u2010inflammatory and antimicrobial activities of phytochemicals from broccoli wastes","volume":"6","author":"Ramesh A","year":"2023","journal-title":"J Adv Biotechnol Exp Ther"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3746\/pnf.2015.20.1.8"},{"key":"e_1_2_7_34_1","first-page":"1299","article-title":"Phenolic composition, antioxidant capacity and antibacterial activity of selected Irish brassica vegetables","volume":"6","author":"Jaiswal AK","year":"2011","journal-title":"Nat Prod Commun"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2011.02829.x"},{"key":"e_1_2_7_36_1","first-page":"100","article-title":"In vitro antibacterial activities of broccoli against foodborne bacteria","volume":"3","author":"Sibi G","year":"2013","journal-title":"J Appl Pharm Sci"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2014.1801"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2015.01.051"},{"key":"e_1_2_7_39_1","first-page":"1","article-title":"Determination of antioxidant compounds, antibacterial activity and minerals content of broccoli","volume":"15","author":"Al\u2010Altaie DMA","year":"2021","journal-title":"Indian J Forensic Med Toxicol"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.02.002"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-011-9330-1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.201000407"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/09637480802702015"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.70718","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.70718","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.70718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T19:21:54Z","timestamp":1783452114000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.70718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,13]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,8,30]]}},"alternative-id":["10.1002\/jsfa.70718"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.70718","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,13]]},"assertion":[{"value":"2025-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}