{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T06:06:55Z","timestamp":1789106815166,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009075","name":"Guangdong Academy of Sciences","doi-asserted-by":"publisher","award":["2023GDASQNRC\u20100201"],"award-info":[{"award-number":["2023GDASQNRC\u20100201"]}],"id":[{"id":"10.13039\/501100009075","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Camellia oleifera<\/jats:italic>\n Abel is extensively cultivated for edible oil, generating abundant leaves, shells, and branches as by\u2010products. However, the bioactivity of polysaccharides from these different tissues remains poorly characterized. To valorize these agricultural wastes, six crude polysaccharides were extracted from the leaves, shells, and branches of\n C. oleifera<\/jats:italic>\n using hot water extraction (HWE) and ultrasound\u2010assisted hot water extraction (USHWE). Their physicochemical properties were compared, and\n in vitro<\/jats:italic>\n anti\u2010inflammatory effects were evaluated to identify the most promising fraction. The active polysaccharide was purified, and its\n in vivo<\/jats:italic>\n anti\u2010aging potential was investigated in\n Caenorhabditis elegans<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Physicochemical characterization revealed distinct structural and compositional differences among the six polysaccharides. Notably, the polysaccharide obtained from branches by HWE (HWE\u2010Branch), which possessed a high total sugar content (35.35%), showed the most potent suppression of lipopolysaccharide (LPS)\u2010induced interleukin\u20106 (IL\u20106) and interleukin\u20101\n \u03b2<\/jats:italic>\n (IL\u20101\n \u03b2<\/jats:italic>\n ) expression in RAW264.7 macrophages, exceeding the effect of dexamethasone. This fraction was purified to yield a homogeneous polysaccharide designated\n C. oleifera<\/jats:italic>\n branch polysaccharide (CBP). In\n C. elegans<\/jats:italic>\n , CBP extended lifespan in a concentration\u2010dependent manner; at 4\u2009mg mL\n \u22121<\/jats:sup>\n , the median lifespan increased by 32.6% compared with the control. Under cyclic heat stress, 4\u2009mg mL\n \u22121<\/jats:sup>\n CBP maintained 38.38% survival after four cycles, whereas all control nematodes died. Furthermore, CBP significantly reduced intracellular reactive oxygen species (ROS) and lipofuscin levels. At 4\u2009mg mL\n \u22121<\/jats:sup>\n , ROS fluorescence intensity decreased from 48.84% to 21.79% and lipofuscin from 25.03% to 10.23%.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Purified\n C. oleifera<\/jats:italic>\n branch polysaccharide CBP showed strong anti\u2010aging effects in\n C. elegans<\/jats:italic>\n by scavenging ROS, attenuating lipofuscin accumulation, and enhancing stress resistance. These findings indicate that CBP is a promising natural ingredient for anti\u2010aging interventions and support the valorization of\n C. oleifera<\/jats:italic>\n processing by\u2010products. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.70953","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:23:56Z","timestamp":1789104236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antioxidant and anti\u2010aging effects of polysaccharides extracted from\n \n Camellia oleifera<\/i>\n <\/scp>\n branches"],"prefix":"10.1002","author":[{"given":"Shengjia","family":"Lu","sequence":"first","affiliation":[{"name":"College of Future Biomass South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianghui","family":"Xin","sequence":"additional","affiliation":[{"name":"College of Future Biomass South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligui","family":"Cheng","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology Guangdong Academy of Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanghui","family":"Ou","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology Guangdong Academy of Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenang","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Future Biomass South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology Guangdong Academy of Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliang","family":"Yao","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Institute of Zoology Guangdong Academy of Sciences Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4948-4176","authenticated-orcid":false,"given":"Litao","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Future Biomass South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.ijbiomac.2012.05.033","article-title":"Antioxidant and antitumor activities of the 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