{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T08:27:29Z","timestamp":1786782449277,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n myo<\/jats:italic>\n \u2010Inositol (MI) is a cyclitol involved in cellular metabolism and phytate degradation, yet its influence on the gastrointestinal tract (GIT) microbiota of laying hens remains poorly understood. This study investigated the effects of graded dietary MI supplementation on the active GIT microbial communities of two commercial laying hen strains.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Lohmann Brown Classic (LB) and Lohmann LSL\u2010Classic (LSL) hens were fed diets supplemented with 0, 1, 2, or 3\u2009g MI\u2009kg\n \u22121<\/jats:sup>\n feed. Active microbiota were assessed by 16S rRNA gene amplicon sequencing (cDNA\u2010based) of 623 mucosa and digesta samples from the crop, duodenum, ileum, and ceca. Microbial community composition was primarily shaped by GIT section (\n R<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.43), followed by host strain (\n R<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.03) and sample type (\n R<\/jats:italic>\n 2<\/jats:sup>\n \u2009=\u20090.02). MI supplementation explained a smaller proportion of variance but significantly influenced specific taxa, particularly in the crop and ceca. Overall, 66 amplicon sequence variants, including members of\n Limosilactobacillus<\/jats:italic>\n ,\n Bacteroides<\/jats:italic>\n , and\n Roseburia<\/jats:italic>\n , differed across MI concentrations. Strain\u2010dependent responses were observed, with LB hens showing greater separation between mucosa and digesta communities, whereas LSL hens exhibited a higher number of MI\u2010responsive amplicon sequence variants in the duodenal and ileal digesta. Correlation analyses revealed associations between\n Ligilactobacillus<\/jats:italic>\n and egg number and yolk MI concentration, and between\n Alistipes<\/jats:italic>\n and egg weight.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Dietary MI supplementation induces localized and strain\u2010dependent alterations in the active GIT microbiota of laying hens. Although these effects were modest compared with host genotype and GIT section, the findings provide first evidence for MI\u2013microbiota interactions with potential relevance to egg production traits. \u00a9 2026 The Author(s).\n Journal of the Science of Food and Agriculture<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.70984","type":"journal-article","created":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T08:24:49Z","timestamp":1786782289000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["myo<\/i>\n \u2010Inositol's role in laying hens\u2019 active gastrointestinal microbiota: a strain comparison"],"prefix":"10.1002","author":[{"given":"Naomi","family":"Sarpong","sequence":"first","affiliation":[{"name":"Institute of Animal Science University of Hohenheim Stuttgart 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