{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T04:25:13Z","timestamp":1787718313574,"version":"build-2784847793"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100019397","name":"University Grants Commission - South Eastern Regional Office","doi-asserted-by":"publisher","award":["F 30\u2010599\/2021\/BSR"],"award-info":[{"award-number":["F 30\u2010599\/2021\/BSR"]}],"id":[{"id":"10.13039\/501100019397","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017531","name":"Gandhi Institute of Technology and Management","doi-asserted-by":"publisher","award":["F. No 2022\/0184"],"award-info":[{"award-number":["F. No 2022\/0184"]}],"id":[{"id":"10.13039\/100017531","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Single\u2010cell protein (SCP) derived from microbial systems is gaining attention as a sustainable alternative protein source for food applications. However, the influence of food\u2010derived substrates on protein accumulation and molecular characteristics in\n Lactiplantibacillus plantarum<\/jats:italic>\n \u2010derived SCP remains poorly understood. Therefore, this study investigated substrate\u2010dependent molecular profiling of SCP produced using fermented rice water and paneer extract through biomass evaluation, protein quantification, sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE) analysis, and liquid chromatography electrospray ionization tandem mass spectrometry (LC\u2010ESI\u2010MS\/MS)\u2010based molecular fingerprinting.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Paneer extract supported the highest viable cell count (2.43\u2009\u00d7\u200910\n 6<\/jats:sup>\n \u2009CFU\u2009mL\n \u22121<\/jats:sup>\n ) and highest mean wet biomass yield (2.82\u2009g\u2009L\n \u22121<\/jats:sup>\n ), whereas fermented rice water resulted in the highest total protein concentration (1.000\u2009g\u2009L\n \u22121<\/jats:sup>\n ) and protein productivity (0.0417\u2009g\u2009L\n \u22121<\/jats:sup>\n \u2009h\n \u22121<\/jats:sup>\n ). SDS\u2010PAGE analysis revealed enhanced electrophoretic band intensity within the 25\u201375\u2009kDa range in rice\u2010derived SCP samples. Comparative LC\u2010ESI\u2010MS\/MS profiling demonstrated conserved chromatographic architectures together with substrate\u2010responsive variations in retention\u2010region distribution and molecular heterogeneity, particularly within intermediate\u2010 and late\u2010eluting regions. Statistical analysis confirmed significant differences among treatments (\n P<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n The divergence between wet biomass production and total protein accumulation indicates that substrate composition differentially regulates metabolic allocation in\n L. plantarum<\/jats:italic>\n \u2010derived SCP systems. Fermented rice water promoted greater total protein enrichment despite lower biomass formation, suggesting substrate\u2010specific biochemical modulation. LC\u2013MS\u2010based molecular fingerprinting further demonstrated that food\u2010derived substrates influence molecular distribution behavior and compositional heterogeneity within SCP biomass. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71015","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T04:16:05Z","timestamp":1787717765000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative molecular profiling of\n \n Lactiplantibacillus plantarum<\/i>\n <\/scp>\n \u2010derived single\u2010cell protein produced from food\u2010derived substrates"],"prefix":"10.1002","author":[{"given":"Koganti","family":"Taarana","sequence":"first","affiliation":[{"name":"Department of Life Sciences GITAM School of Science Visakhapatnam 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