{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:16:08Z","timestamp":1788153368356,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Due to weak stability and bioavailability,\n                      <jats:italic>\u03b3<\/jats:italic>\n                      \u2010aminobutyric acid (GABA) and naringenin (Nar), two agents with sleep\u2010promoting activities, are limited in practical application. To improve their sleep\u2010promoting efficacy, this study developed a stability\u2010enhanced co\u2010loaded liposomal system (GABA\u2010Nar\u2010Lip) as a lyophilized powder.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The non\u2010lyophilized GABA\u2010Nar\u2010Lip exhibited good dispersibility, with an average particle size of 161.34\u2009\u00b1\u20091.68\u2009nm, and achieved encapsulation efficiencies of 46.18\u2009\u00b1\u20090.32% (GABA) and 82.01\u2009\u00b1\u20091.33% (Nar), respectively. The stability of GABA\u2010Nar\u2010Lip was significantly enhanced after lyophilization with sugars (especially 0.10\u2009g\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      trehalose) as cryoprotectants (CPAs), and maintained targeted release in the small intestine. The CPAs exerted cryoprotective effects by replacing water molecules and forming hydrogen bonds with soy phosphatidylcholine, thereby preserving the spherical structure and unilamellar vesicle morphology of the liposomes. In caffeine\u2010induced zebrafish models, lyophilized GABA\u2010Nar\u2010Lip showed an inferior but still significant effect in sleep latency reduction. Moreover, it decreased nocturnal twitching activity and enhanced daytime locomotor activity on the following day, without the adverse effects associated with melatonin, such as poor sleep quality and reduced daytime activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>The stabilized lyophilized GABA\u2010Nar\u2010Lip powder is a promising, safe sleep aid with balanced efficacy and no compromise to next\u2010day performance, supporting its potential application in dietary sleep interventions. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71017","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:23:41Z","timestamp":1788150221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stability\u2010enhanced liposomal system co\u2010loaded with\n                    <i>\u03b3<\/i>\n                    \u2010aminobutyric acid and naringenin: outstanding sleep\u2010promoting efficacy"],"prefix":"10.1002","author":[{"given":"Ming","family":"Wu","sequence":"first","affiliation":[{"name":"School of Food Science and Engineering, Guangdong Provincial Key Laboratory of Green Processing of Natural Products and Product Safety South China University of Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering, Guangdong Provincial Key Laboratory of Green 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