{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:06:46Z","timestamp":1788934006442,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Constipation is a common gastrointestinal disorder. Although conventional treatments can alleviate symptoms, they often cause side effects.\n Bifidobacterium<\/jats:italic>\n , a safe and well\u2010tolerated probiotic, has demonstrated potential in constipation management; however, functional variability among strains limits its broad application.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n This study investigated the efficacy of four novel\n Bifidobacterium<\/jats:italic>\n strains and the commercial reference\n Bifidobacterium animalis<\/jats:italic>\n subsp.\n lactis<\/jats:italic>\n BB12 using a loperamide hydrochloride (LOP)\u2010induced constipated mouse model.\n Bifidobacterium<\/jats:italic>\n supplementation significantly improved constipation phenotypes, including increased fecal water content, intestinal transit rate, and fecal output, while shortening the time to first black stool. It also enhanced the intestinal barrier by up\u2010regulating tight junction proteins, leading to reduced tumor necrosis factor alpha (TNF\u2010\u03b1), interleukin\u20101 beta (IL\u20101\u03b2), and elevated anti\u2010inflammatory interleukin 10 (IL\u201010) and fecal short\u2010chain fatty acids (SCFAs).\n Bifidobacterium<\/jats:italic>\n modulated gut microbiota composition, with\n B. animalis<\/jats:italic>\n subsp.\n lactis<\/jats:italic>\n C\u20102 notably enriching\n Actinobacteriota<\/jats:italic>\n , which correlated positively with constipation relief and SCFAs, and negatively with inflammation. Moreover, the\n abfA<\/jats:italic>\n cluster expression in C\u20101 and C\u20102 was similar to BB12 and higher than in other strains, which correlated with the superior performance of\n B. animalis<\/jats:italic>\n strains in constipation alleviation, suggesting a potential genetic determinant warranting further functional validation.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n These findings reveal strain\u2010specific mechanisms and support the targeted use of\n Bifidobacterium<\/jats:italic>\n for constipation treatment. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71070","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:29:31Z","timestamp":1788931771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Strain\u2010dependent effects of\n \n Bifidobacterium<\/i>\n <\/scp>\n on loperamide\u2010induced constipation via gut microbiota and\n \n abfA<\/i>\n <\/scp>\n cluster"],"prefix":"10.1002","author":[{"given":"Xiaoyan","family":"Song","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Food Quality and Health, College of Food Science and Engineering Tianjin University of Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Classykiss Yogurt (Suzhou) Co., Ltd Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Quality and Health, College of Food Science and Engineering Tianjin University of Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Classykiss Yogurt (Suzhou) Co., Ltd Suzhou 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Laboratory of Food Quality and Health, College of Food Science and Engineering Tianjin University of Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3400-5531","authenticated-orcid":false,"given":"Longgang","family":"Jia","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Quality and Health, College of Food Science and Engineering Tianjin University of Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41579-020-0433-9","article-title":"Gut microbiota in human metabolic health and disease","volume":"19","author":"Fan Y","year":"2021","journal-title":"Nat Rev 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