{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:04:34Z","timestamp":1789563874729,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004770","name":"Universit\u00e0 degli Studi di Parma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004770","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Antibiotic\u2010free production within the meat supply chain is a source of concern since new voluntary label claims need to be authenticated to prevent fraud. Therefore, an untargeted\n                      <jats:sup>1<\/jats:sup>\n                      H NMR\u2010based metabolomics approach coupled to unsupervised (principal component analysis) and supervised (orthogonal partial least squares discriminant analysis) multivariate analyses was applied to kidney (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200947) and diaphragm (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200954) samples of pigs from four Italian fattening farms to investigate changes in polar and nonpolar metabolite levels and differentiate between pigs treated with antibiotics (ABT) and pigs never exposed to antibiotic administration (ABF).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The findings revealed a full discrimination between ABF and ABT pig samples, particularly when considering the aqueous extract of the investigated tissues. All discriminating metabolites differently accumulated in the polar extracts were annotated and grouped according to their molecular structure under the different biochemical categories (carbohydrates, amino acids and derivatives, organic acids, alcohols, choline and derivatives, and nucleosides) to discuss the putative biological function of the metabolism involved in response to antibiotic administration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      Among the biochemical categories listed, amino acids and derivatives represented most (60%) of the affected metabolites in relation to antibiotic administration. \u00a9 2026 The Author(s).\n                      <jats:italic>Journal of the Science of Food and Agriculture<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71074","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:15:57Z","timestamp":1789560957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomic insights for the authentication and traceability of antibiotic\u2010free pork: a pilot study"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9068-0254","authenticated-orcid":false,"given":"Maria Olga","family":"Varr\u00e0","sequence":"first","affiliation":[{"name":"Department of Food and Drug University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Pia","family":"Fabrile","sequence":"additional","affiliation":[{"name":"Department of Food and Drug University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ghidini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences University of Milan  Lodi Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8568-6851","authenticated-orcid":false,"given":"Augusta","family":"Caligiani","sequence":"additional","affiliation":[{"name":"Department of Food and Drug University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Scali","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia\u2010Romagna  Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Lolli","sequence":"additional","affiliation":[{"name":"Department of Food and Drug University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni Loris","family":"Alborali","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia\u2010Romagna  Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Ianieri","sequence":"additional","affiliation":[{"name":"Department of Food and Drug University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2253-3647","authenticated-orcid":false,"given":"Emanuela","family":"Zanardi","sequence":"additional","affiliation":[{"name":"Department of Food and Drug University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-022-00136-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12091776"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/tas\/txac063"},{"key":"e_1_2_9_5_1","first-page":"29","article-title":"Regulation (EC) No. 1831\/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition","volume":"268","year":"2003","journal-title":"OJEU"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Commission Regulation (EU) No. 37\/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin","volume":"15","year":"2010","journal-title":"OJEU"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.appet.2019.104577"},{"key":"e_1_2_9_8_1","unstructured":"CWA 17369 Authenticity and fraud in the feed and food chain \u2013 concepts terms and definitions. CEN European Committee for Standardization & CEN\u2010CENELEC Management Centre(2019)."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2010.06.001"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics6040034"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25143265"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2533\/chimia.2016.329"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.133856"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-100220-9.00007-2"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2022.109488"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c05163"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12234259"},{"key":"e_1_2_9_18_1","first-page":"1","article-title":"Commission Regulation (EC) No. 1107\/96 of 12 June 1996 on the registration of geographical indications and designations of origin under the procedure laid down in article 17 of Council Regulation (EEC) No. 2081\/92","volume":"148","year":"1996","journal-title":"OJEU"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9120892"},{"key":"e_1_2_9_20_1","unstructured":"ClassyFarm \u2013 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia\u2010Romagna \u2018BRUNO UBERTINI\u2019Available:https:\/\/www.classyfarm.it\/[10 September 2025]."},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.120855"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo2040775"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1mb05023k"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-016-1105-7"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfaa023"},{"key":"e_1_2_9_27_1","unstructured":"HMDB Human Metabolome DatabaseAvailable:https:\/\/hmdb.ca\/[12 November 2025]."},{"key":"e_1_2_9_28_1","unstructured":"BMRB Biological Magnetic Resonance BankAvailable:https:\/\/bmrb.io\/[12 November 2025]."},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2016.07.004"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202000052"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.animal.2024.101393"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo15080555"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.853995"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0264953"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2020.108374"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.737903"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.925846"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00567"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00883"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2026.115024"},{"key":"e_1_2_9_41_1","first-page":"31","volume-title":"Manipulating Pig Production XIII, Proceedings of the Thirteenth Biennial Conference of the Australasian Pig Science Association","author":"Rakhshandeh A","year":"2011"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1054\/clnu.2001.0402"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114513001955"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-020-02592-0"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c03651"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2912-8"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-45328-2_5"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-023-03650-z"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-03107-1"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71074","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.71074","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71074","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:16:01Z","timestamp":1789560961000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.71074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":48,"alternative-id":["10.1002\/jsfa.71074"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.71074","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]},"assertion":[{"value":"2026-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jsfa.71074"}}