{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:04:39Z","timestamp":1790057079832,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Postharvest soft rot caused by\n                      <jats:italic>Rhizopus stolonifer<\/jats:italic>\n                      results in severe economic losses globally. Current control strategies rely heavily on synthetic fungicides, raising environmental and food safety concerns. This study evaluated the synergistic antifungal potential of methyl salicylate (MeSA) combined with 2,3\u2010butanedione, nerol, clove essential oil, or oregano essential oil, and elucidated the underlying antifungal mechanism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Screening via the co\u2010toxicity factor method revealed that the combination of MeSA (41.7\u2009\u03bcL\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) and 2,3\u2010butanedione (37.4\u2009\u03bcL\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) exhibited significant synergism (\n                      <jats:italic>F<\/jats:italic>\n                      \u2009=\u200934.59), allowing for reduced dosages while maintaining high efficacy.\n                      <jats:italic>In vivo<\/jats:italic>\n                      assays demonstrated that this combination (83.4 and 74.8\u2009\u03bcL\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) significantly suppressed disease incidence, reducing soft rot rates to 11.11% in figs and 8.33% in tomatoes, while markedly inhibiting lesion expansion in peaches and green peppers. Mechanistically, the treatment compromised\n                      <jats:italic>R. stolonifer<\/jats:italic>\n                      viability by disrupting membrane integrity and permeability, inducing oxidative stress and mitochondrial dysfunction, which ultimately triggered apoptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      The combination of\n                      <jats:styled-content style=\"fixed-case\">MeSA<\/jats:styled-content>\n                      and 2,3\u2010butanedione offers a potent, eco\u2010friendly bio\u2010rational strategy for managing postharvest soft rot, presenting a viable alternative to conventional synthetic fungicides for fruit preservation. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71083","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:29:16Z","timestamp":1790054956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic antifungal activity of methyl salicylate and 2,3\u2010butanedione against\n                    <scp>\n                      <i>Rhizopus stolonifer<\/i>\n                    <\/scp>\n                    : membrane disruption mechanism and control of postharvest soft rot"],"prefix":"10.1002","author":[{"given":"Wang","family":"Xiaoyu","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering, Shandong Agriculture and 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