{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:45:23Z","timestamp":1791985523408,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The large volume of supernatant generated during\n                      <jats:italic>Grifola frondosa<\/jats:italic>\n                      extraction is typically discarded, resulting in substantial resource waste and environmental pressure. Such agro\u2010fungal processing by\u2010products are rich in potentially bioactive macromolecules, but efficient recycling strategies and biofunctional verification remain limited. Meanwhile, safe natural antiviral ingredients for pediatric enterovirus infection are lacking. This study established a sustainable chromatographic bioprocess to recover a low\u2010molecular\u2010weight polysaccharide\u2010peptide (LMWPP) from\n                      <jats:italic>G. frondosa<\/jats:italic>\n                      waste liquid, with the aims of achieving high\u2010value circular utilization of fungal processing wastewater and exploring its anti\u2010EV71 potential.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Purified LMWPP with an average molecular weight of 1.252\u2009\u00d7\u200910\n                      <jats:sup>4<\/jats:sup>\n                      \u2009Da possessed pyranose structures with\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2010 and\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010glycosidic bonds and consisted of multiple monosaccharides and leucine\u2010dominant amino acids. Recovered LMWPP exerted marked anti\u2010EV71 effects by mitigating cytotoxicity, inhibiting viral replication, downregulating VP1 mRNA expression, and alleviating cell apoptosis. Transcriptomic analysis further demonstrated its regulatory effects on the complement and coagulation cascades, peroxisome proliferator\u2010activated receptor (PPAR) signaling, and oxidative phosphorylation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This integrated separation strategy enables efficient valorization of\n                      <jats:italic>G. frondosa<\/jats:italic>\n                      liquid by\u2010products and clarifies the structure\u2013activity relationship of LMWPP. The recycled natural LMWPP shows promise as a pediatric antiviral functional food ingredient, providing a feasible industrial approach for high\u2010value reuse of edible fungal processing waste. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71122","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:51:19Z","timestamp":1791982279000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Valorization of\n                    <scp>\n                      <i>Grifola frondosa<\/i>\n                    <\/scp>\n                    processing by\u2010products: purification, structural characterization, and anti\u2010\n                    <scp>EV71<\/scp>\n                    bioactivity of a novel low\u2010molecular\u2010weight polysaccharide\u2010peptide"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4454-075X","authenticated-orcid":false,"given":"Wenyu","family":"Xiong","sequence":"first","affiliation":[{"name":"College of Food Science Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"School of Food Science and Engineering South China University of Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiang","family":"He","sequence":"additional","affiliation":[{"name":"College of Food Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Food Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Food Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6219-3093","authenticated-orcid":false,"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"National Engineering Research Center of JUNCAO Technology  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Food Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2014.11.035"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.12.003"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO01116H"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.10.253"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.02.034"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0FO03327H"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.01.090"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.744"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2017.1247891"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.08.020"},{"key":"e_1_2_10_12_1","article-title":"Polysaccharide\u2010peptide from Trametes versicolor: the potential medicine for colorectal cancer treatment","volume":"10","author":"He ZC","year":"2022","journal-title":"Biomedicine"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2013.01.006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2009.03.013"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2010.02.006"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1139\/o96-010"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112339"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.13332"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.624536"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.02.009"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00563-09"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2022.2147961"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.12.005"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1088\/1755-1315\/947\/1\/012041"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2018.06.077"},{"key":"e_1_2_10_26_1","first-page":"1058","article-title":"Isolation, purification and structural analysis of GL\u2010PP\u20103A, an active polysaccharide peptide from Ganoderma lucidum","volume":"42","author":"Wang SZ","year":"2007","journal-title":"Acta Pharm Sin"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.07.035"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9639-1_30"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-018-0500-x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202200766"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85421-0"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2010.08.014"},{"key":"e_1_2_10_33_1","article-title":"Beta\u2010elimination for release of O\u2010GalNAc\u2010linked oligosaccharides from glycoproteins and glycopeptides","volume":"17","author":"Fukuda M","year":"2001","journal-title":"Curr Protoc Mol Biol Ch"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.10.216"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.07.010"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2006.03.039"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490360609"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2016.07.014"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2013.02.057"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2004.07.027"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2019.08.004"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115279"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-09070-2"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.02.017"},{"key":"e_1_2_10_45_1","article-title":"Tissue\u2010specific functional interaction between apolipoproteins A1 and E in cold\u2010induced adipose organ mitochondrial energy metabolism","volume":"1866","author":"Xepapadaki E","year":"2021","journal-title":"BBA\u2010Mol Cell Biol L"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1086\/525287"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.5.2418-2428.2006"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.11.497"},{"key":"e_1_2_10_49_1","first-page":"103","article-title":"Mutations in mitochondrial NADH dehydrogenase subunit 1 (mtND1) gene in colorectal carcinoma","volume":"32","author":"Yusnita Y","year":"2010","journal-title":"Malays J Pathol"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290222"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b05802"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71122","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.71122","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:51:21Z","timestamp":1791982281000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.71122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":50,"alternative-id":["10.1002\/jsfa.71122"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.71122","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"2026-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jsfa.71122"}}