{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:54:12Z","timestamp":1791262452091,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Calcium deficiency is a widespread concern affecting individuals across all age groups and countries. Curd offers a promising way to fortify nutrients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODOLOGY<\/jats:title>\n                    <jats:p>\n                      In order to make calcium\u2010enriched curd as a simple cost\u2010effective dietary intervention to increase calcium intake, this study developed a novel calcium curd strip (CCS). A polyvinylidene fluoride (PVDF) membrane with immobilized curd bacteria and different calcium carbonate concentrations was used in the CCS. Additionally, Wistar rats were used in\n                      <jats:italic>in vivo<\/jats:italic>\n                      animal investigations to assess calcium bioavailability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The CCS served as both a starting culture and a way to deliver calcium when submerged in warm milk. Calcium levels in the prepared curd were ranging from 117\u2009mg 100\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      to 519\u2009mg 100\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , depending on the amount of calcium incorporated into the strip. The physicochemical characteristics of curd, such as pH, lactic acid concentration, and syneresis, were similar to those of curd made traditionally, suggesting comparable quality. Microbiological analysis, verified the absence of moulds, yeast, and coliforms. When compared with different conditions of calcium consumption such as traditional curd, curd strip, or regular diet, rats fed CCS\u2010based curd showed noticeably higher blood calcium levels (10.56\u2009\u00b1\u20090.49\u2009mg dL\n                      <jats:sup>\u22121<\/jats:sup>\n                      in comparison to control having 8.56\u2009\u00b1\u20090.23\u2009mg dL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) and more calcium deposition in bone (58.86\u2009\u00b1\u20091.53\u2009mg\u2009g\n                      <jats:sup>\u22121<\/jats:sup>\n                      in comparison to control having 25.93\u2009\u00b1\u20095\u2009mg\u2009g\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) in 4\u2009weeks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>The developed CCS is a useful and efficient method for creating calcium\u2010enriched curd with increased bioavailability. The technique provides a practical way to maintain bone health and increase dietary calcium consumption, which improves nutritional security. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71125","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:24:14Z","timestamp":1791260654000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Elevating dairy delights and nutritional security with calcium enrichment using an innovative calcium curd strip (\n                    <scp>CCS<\/scp>\n                    ) validated through animal studies"],"prefix":"10.1002","author":[{"given":"Maheshawari J","family":"Behere","sequence":"first","affiliation":[{"name":"Halophyte Biology, Biotechnology and Marine Environment Division CSIR \u2013 Central Salt and Marine Chemicals Research Institute  Bhavnagar India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruchita","family":"Bhomia","sequence":"additional","affiliation":[{"name":"Institute of Science, Nirma University  Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shruti","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Institute of Science, Nirma University  Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jigna","family":"Shah","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Nirma University  Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhruvi","family":"Vejpara","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Nirma University  Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9989-8137","authenticated-orcid":false,"given":"Soumya","family":"Haldar","sequence":"additional","affiliation":[{"name":"Halophyte Biology, Biotechnology and Marine Environment Division CSIR \u2013 Central Salt and Marine Chemicals Research Institute  Bhavnagar India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.2147\/CIA.S157523","article-title":"The good, the bad, and the ugly of calcium supplementation: a review of calcium intake on human health","volume":"28","author":"Li K","year":"2018","journal-title":"Clin Interv Aging"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"e13093","DOI":"10.1016\/j.heliyon.2023.e13093","article-title":"Modified eggshell powder using thermal treatment and its application in Ca\u2010fortified dog biscuits","volume":"9","author":"Therdthai N","year":"2023","journal-title":"Heliyon"},{"key":"e_1_2_11_4_1","unstructured":"ShliskyJ MandlikR AskariS AbramsS BelizanJM BourassaMWet al. Calcium Deficiency Worldwide: Prevalence of Inadequate Intakes and Associated Health Outcomes(2022)."},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1007\/s00198-015-3440-3","article-title":"The National Osteoporosis Foundation's position statement on peak bone mass development and lifestyle factors: a systematic review and implementation recommendations","volume":"27","author":"Weaver CM","year":"2016","journal-title":"Osteoporos Int"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2022.111212","article-title":"Fortification\/enrichment of milk and dairy products by encapsulated bioactive ingredients","volume":"1","author":"Adinepour F","year":"2022","journal-title":"Food Res Int"},{"key":"e_1_2_11_7_1","unstructured":"https:\/\/www.who.int\/health-topics\/micronutrient"},{"key":"e_1_2_11_8_1","unstructured":"http:\/\/ods.od.nih.gov"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1094\/CCHEM-11-14-0225-R","article-title":"Influence of oxalate, phytate, tannin, dietary fiber, and cooking on calcium bioavailability of commonly consumed cereals and millets in India","volume":"92","author":"Amalraj A","year":"2015","journal-title":"Cereal Chem"},{"key":"e_1_2_11_10_1","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.583654","article-title":"Modern India and dietary calcium deficiency\u2014half a century nutrition data\u2014retrospect\u2013introspect and the road ahead","volume":"12","author":"Harinarayan CV","year":"2021","journal-title":"Front Endocrinol"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.1017\/S1368980020002499","article-title":"Severe acute malnutrition, calcium and vitamin D: important interactions","volume":"23","author":"Witte MA","year":"2020","journal-title":"Public Health Nutr"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1039\/D3FB00079F","article-title":"Food fortification in India as malnutrition concern: a global approach","volume":"1","author":"Thakur S","year":"2023","journal-title":"Sust Food Technol"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","first-page":"84","DOI":"10.9734\/ejnfs\/2025\/v17i51710","article-title":"From tradition to innovation: a review of Misti Dahi (sweetened curd) as a probiotic\u2010rich functional dairy product","volume":"17","author":"Das B","year":"2025","journal-title":"Eur J Nutr Food Saf"},{"key":"e_1_2_11_14_1","first-page":"1","article-title":"Therapeutic uses of curd: a review","volume":"13","author":"Das SP","year":"2019","journal-title":"IOSR J Environ Sci"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","DOI":"10.3389\/fnut.2022.1058238","article-title":"Association between probiotic, prebiotic, and yogurt consumption and chronic kidney disease: the NHANES 2010\u20132020","volume":"23","author":"Liu X","year":"2022","journal-title":"Front Nutr"},{"key":"e_1_2_11_16_1","first-page":"123","volume-title":"International Conference Sustainable Environmental Engineering and Science","author":"Dutta A","year":"2021"},{"key":"e_1_2_11_17_1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1007\/s12603-015-0498-8","article-title":"Fortification of yogurts with vitamin D and calcium enhances the inhibition of serum parathyroid hormone and bone resorption markers: a double blind randomized controlled trial in women over 60 living in a community dwelling home","volume":"19","author":"Bonjour JP","year":"2015","journal-title":"J Nutr Health Aging"},{"key":"e_1_2_11_18_1","doi-asserted-by":"crossref","first-page":"23229","DOI":"10.3402\/fnr.v58.23229","article-title":"A comparative study of calcium absorption following a single serving administration of calcium carbonate powder versus calcium citrate tablets in healthy premenopausal women","volume":"58","author":"Wang H","year":"2014","journal-title":"Food Nutr Res"},{"key":"e_1_2_11_19_1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.2147\/TCRM.S3552","article-title":"The use of calcium and vitamin D in the management of osteoporosis","volume":"4","author":"Sunyecz JA","year":"2008","journal-title":"Ther Clin Risk Manag"},{"key":"e_1_2_11_20_1","volume-title":"StatPearls [Internet]","author":"Fritz K","year":"2023"},{"key":"e_1_2_11_21_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1007\/s11926-008-0020-y","article-title":"Calcium and vitamin D: skeletal and extraskeletal health","volume":"10","author":"Khazai N","year":"2008","journal-title":"Curr Rheumatol Rep"},{"key":"e_1_2_11_22_1","doi-asserted-by":"crossref","first-page":"3492","DOI":"10.1007\/s13197-021-05339-3","article-title":"A novel technique for the preparation of curd (Dahi) by PVDF membrane\u2010based strip","volume":"59","author":"Kumar SB","year":"2022","journal-title":"J Food Sci Technol"},{"key":"e_1_2_11_23_1","unstructured":"My Agriculture Information Bankhttp:\/\/www.agriinfo.in\/default.aspx?page=topic&superid=&opicid=716[20 October 2020]."},{"key":"e_1_2_11_24_1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.1471-0307.2006.00264.x","article-title":"Syneresis in set yogurt as affected by EPS starter cultures and levels of solids","volume":"59","author":"Amatayakul T","year":"2006","journal-title":"Int J Dairy Technol"},{"key":"e_1_2_11_25_1","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.tifs.2020.07.013","article-title":"Analytical advances in the determination of calcium in bovine milk, dairy products and milk\u2010based infant formulas","volume":"103","author":"Masotti F","year":"2020","journal-title":"Trends Food Sci Technol"},{"key":"e_1_2_11_26_1","first-page":"36","article-title":"The content of macro and trace elements in curd and traditional white brined cheese","volume":"75","author":"Levkov V","year":"2017","journal-title":"Vet Zootec"},{"key":"e_1_2_11_27_1","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1080\/09637486.2016.1227306","article-title":"Eggshell fractions containing different particle sized affect mineral absorption but not bone mineral retention in growing rats","volume":"68","author":"Milbradt BG","year":"2017","journal-title":"Int J Food Sci Nutr"},{"key":"e_1_2_11_28_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.33546\/bnj.299","article-title":"Effect of eggshell flour on blood calcium levels in pregnant mice","volume":"3","author":"Safitri IR","year":"2017","journal-title":"Belitung Nursing J"},{"key":"e_1_2_11_29_1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3390\/pr7040178","article-title":"Effect of morphology of calcium carbonate on toughness behavior and thermal stability of epoxy\u2010based composites","volume":"7","author":"Yang G","year":"2019","journal-title":"Processes"},{"key":"e_1_2_11_30_1","first-page":"Academic Press","volume-title":"Profiles of Drug Substances, Excipients and Related Methodology","author":"Al Omari MM","year":"2016"},{"key":"e_1_2_11_31_1","first-page":"845","article-title":"Molecular identification of lactic acid bacteria with probiotic potential from local curd","volume":"15","author":"Hassanzadeh Golijan M","year":"2023","journal-title":"J Chem Health Risks"},{"key":"e_1_2_11_32_1","volume-title":"Dietary Reference Intakes for Calcium and Vitamin D","author":"Valle HB","year":"2011"},{"key":"e_1_2_11_33_1","first-page":"1460","article-title":"Microbial interactions between lactobacillus bulgaricus and Streptococcus thermophilus in milk","volume":"8","author":"Mchiouer K","year":"2017","journal-title":"J Mater Environ Sci"},{"key":"e_1_2_11_34_1","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1177\/10820132231193479","article-title":"Effect of pulsed electric field treated on quality of curd","volume":"30","author":"Preeti B","year":"2024","journal-title":"Food Sci Technol Int"},{"key":"e_1_2_11_35_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jfca.2023.105475","article-title":"Elemental analysis and health risk assessment of different hazelnut varieties (Corylus avellana L.) collected from Giresun\u2010Turkey","volume":"122","author":"\u015eeker ME","year":"2023","journal-title":"J Food Compos Anal"},{"key":"e_1_2_11_36_1","first-page":"693","article-title":"A study on quality of curd produced from different types of milk","volume":"7","author":"Sharmila S","year":"2018","journal-title":"World J Pharm Res"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.71125","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:24:28Z","timestamp":1791260668000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.71125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":35,"alternative-id":["10.1002\/jsfa.71125"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.71125","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]},"assertion":[{"value":"2026-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jsfa.71125"}}