{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:51:37Z","timestamp":1791348697115,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Rhaponticoides mykalea<\/jats:italic>\n is an endemic species with limited data on its phytochemistry and biological activity. The aqueous ethanol extract (700\u2009mL\u2009L\n \u22121<\/jats:sup>\n , v\/v) of the aerial parts and its\n n<\/jats:italic>\n \u2010hexane, ethyl acetate (\n RPEtAc<\/jats:styled-content>\n ),\n n<\/jats:italic>\n \u2010butanol, and water fractions were evaluated for enzyme inhibition, antioxidant activity, cytotoxicity, and apoptosis\u2010related effects, together with\n LC<\/jats:styled-content>\n \u2013\n HRMS<\/jats:styled-content>\n profiling and molecular docking\/\n MM<\/jats:styled-content>\n \u2010\n GBSA<\/jats:styled-content>\n analysis.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Among the samples,\n RPEtAc<\/jats:styled-content>\n showed the highest total phenolic (273.49\u2009\u00b1\u20093.80\u2009mg\n GAE<\/jats:styled-content>\n \u2009g\n \u22121<\/jats:sup>\n ) and flavonoid (175.45\u2009\u00b1\u20092.53\u2009mg\u2009CE\u2009g\n \u22121<\/jats:sup>\n ) contents and the strongest\n DPPH<\/jats:styled-content>\n ,\n FRAP<\/jats:styled-content>\n , and\n CUPRAC<\/jats:styled-content>\n activities. It was the most active\n \u03b1<\/jats:italic>\n \u2010glucosidase inhibitor (\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n \u2009=\u2009128.78\u2009\u00b1\u20092.57\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n ), exceeding acarbose (209.21\u2009\u00b1\u20091.32\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n ) under the assay conditions, and the most cytotoxic fraction toward\n RL95<\/jats:styled-content>\n \u20102 endometrial cancer cells (\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n \u2009=\u200915.35\u2009\u00b1\u20090.68\u2009\u03bcg\u2009mL\n \u22121<\/jats:sup>\n , 48\u2009h), increasing caspase\u20103 and caspase\u20109 activities and the Bax\/Bcl\u20102 ratio.\n LC<\/jats:styled-content>\n \u2013\n HRMS<\/jats:styled-content>\n identified phenolic acids (chlorogenic, vanillic, ferulic, and 3\u2010(4\u2010hydroxyphenyl)propionic acids) and flavonoid derivatives.\n In silico<\/jats:italic>\n , flavonoid glycosides detected in\n RPEtAc<\/jats:styled-content>\n (quercetin 3\u2010rutinoside 7\u2010glucoside, narcissin, isoquercitrin) showed favorable predicted\n MM<\/jats:styled-content>\n \u2010\n GBSA<\/jats:styled-content>\n binding at the\n BCL<\/jats:styled-content>\n \u20102\n BH3<\/jats:styled-content>\n groove, and quercetin, narcissin, and rutin at the\n \u03b1<\/jats:italic>\n \u2010glucosidase site.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n The ethyl acetate fraction of\n R. mykalea<\/jats:italic>\n aerial parts is a phenolic\u2010rich fraction with antioxidant,\n \u03b1<\/jats:italic>\n \u2010glucosidase inhibitory, and apoptosis\u2010associated cytotoxic activity demonstrated at the fraction level. The docking results provide computational support only and require experimental validation with isolated compounds. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71136","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:30:58Z","timestamp":1791347458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical profiling and bioactivity of\n \n Rhaponticoides mykalea<\/i>\n <\/scp>\n :\n \u03b1<\/i>\n \u2010glucosidase inhibition, apoptosis\u2010associated cytotoxicity, and\n \n in silico<\/i>\n <\/scp>\n molecular insights"],"prefix":"10.1002","author":[{"given":"Leyla","family":"Pa\u015fayeva","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Erciyes University Kayseri 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