{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:50:28Z","timestamp":1792129828197,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n Overweight induced by a high\u2010fat diet (HFD) is closely associated with dyslipidemia, oxidative stress, intestinal barrier dysfunction, and gut microbiota imbalance. Tea polyphenols have shown metabolic benefits; however, comparative information regarding their biological effects across different intervention doses remains limited. This study evaluated the effects of different doses of green tea polyphenols (GTP) on metabolic disorders in HFD\u2010induced overweight mice.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Green tea polyphenols reduced body weight gain and improved lipid metabolism without affecting food intake, with the strongest overall effects observed in the 300\u2009mg\u2009kg\n \u22121<\/jats:sup>\n group. Compared with the normal control (NC) group, the HFD group showed increased serum total cholesterol (TC) (+104.01%), triglyceride (TG) (+57.06%), and low\u2010density lipoprotein cholesterol (LDL\u2010C) (+64.44%) levels and decreased high\u2010density lipoprotein cholesterol (HDL\u2010C) (\u221241.29%). These lipid disturbances were attenuated significantly by GTP intervention, especially in the 300\u2009mg\u2009kg\n \u22121<\/jats:sup>\n group (\n P<\/jats:italic>\n \u2009<\u20090.05). GTP also alleviated liver injury and oxidative stress, as indicated by decreased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, increased catalase (CAT) and superoxide dismutase (SOD) activities, increased glutathione (GSH) levels, and reduced malondialdehyde (MDA) levels. In addition, GTP intervention was associated with increased colonic goblet cell numbers and changes in the expression of the tight\u2010junction proteins claudin\u20101 and occludin. Gut microbiota analysis revealed a reduced\n Firmicutes\/Bacteroidota<\/jats:italic>\n ratio and enrichment of beneficial bacteria, with the high\u2010dose group showing a microbial profile closer to that of the normal controls.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Green tea polyphenol ameliorated HFD\u2010induced metabolic abnormalities involving lipid metabolism, oxidative stress, and intestinal barrier function, and these improvements were accompanied by marked alterations in gut microbial composition. Among the tested doses, 300\u2009mg\u2009kg\n \u22121<\/jats:sup>\n showed the strongest overall effects. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71137","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:27:37Z","timestamp":1792128457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of three doses of tea polyphenols on lipid metabolism, intestinal barrier function, and gut microbiota in overweight mice"],"prefix":"10.1002","author":[{"given":"Guang\u2010Yin","family":"Lu","sequence":"first","affiliation":[{"name":"China Institute of Cereal & Oil Science and Technology Academy of National Food and Strategic Reserves Administration Beijing China"},{"name":"University of Shanghai for Science and Technology Shanghai 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