{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:50:35Z","timestamp":1791355835614,"version":"4.3.1"},"reference-count":64,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["C2024204134"],"award-info":[{"award-number":["C2024204134"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Levan is a biodegradable and biocompatible microbial polysaccharide with promising food and biomedical applications. However, the low soluble expression of levansucrase (SacB) severely hinders large\u2010scale levan production. This study aimed to enhance SacB expression and explore levan\u2010based functional edible films for fruit preservation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      A VNp6 signal peptide\u2010fused recombinant expression system coupled with bacterial envelope material (BEM) particle immobilization was constructed to realize efficient SacB expression and streamline enzyme purification. Bioinformatic and enzymatic assays verified that the recombinant SacB was a structurally stable soluble protein. The enzyme exhibited optimal catalytic activity at pH\u20095.8 and 50\u2009\u00b0C, affording a levan yield of 21.18\u2009g\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      under optimized conditions. Structural characterization demonstrated that the enzymatically synthesized levan possessed a porous \u03b2\u2010(2\u2009\u2192\u20096)\u2010linked network structure suitable for edible film fabrication. Levan\u2010based composite films with 2\u2009g\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      tea polyphenols exhibited integrated desirable properties, including strong antioxidant activity, broad\u2010spectrum antibacterial ability and favorable mechanical performance, which effectively alleviated fruit spoilage and displayed great potential for berry preservation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This study established an integrated technical system for SacB expression and levan functional utilization. It provides a novel strategy for high\u2010efficiency heterologous SacB expression and offers theoretical and technical support for developing green packaging materials and high\u2010value levan application. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71142","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:01:10Z","timestamp":1791352870000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficient biosynthesis of levan using recombinant levansucrase and preservation efficacy of edible composite films"],"prefix":"10.1002","author":[{"given":"Jingxin","family":"Luo","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Shanxi Normal University  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifang","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingran","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baomei","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Shanxi Normal University  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Sang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6543-0373","authenticated-orcid":false,"given":"Fangkun","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Hebei Agricultural University  Baoding P. R. 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