{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:46:48Z","timestamp":1792043208850,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472308"],"award-info":[{"award-number":["32472308"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Our previous research has revealed that the\n Lactiplantibacillus plantarum<\/jats:italic>\n DPUL\u2010S164 metabolizes tryptophan (Trp) to produce indole\u20103\u2010lactic acid (ILA), indole\u20103\u2010acetic acid (IAA) and indole\u20103\u2010carboxaldehyde (I3C). This study systematically elucidated the molecular regulatory mechanism of this strain for the metabolism of Trp to produce ILA, I3C and IAA through genome\u2010wide functional annotation, bioinformatics analysis, high\u2010performance liquid chromatography, and real\u2010time fluorescence quantitative polymerase chain reaction.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Under Trp\u2010enriched conditions, the three indole derivatives showed significant time\u2010dependent cumulative effects, with ILA biosynthesis exhibiting the most significant up\u2010regulation. Molecular mechanistic studies have shown that the metabolic pathway involves synergistic regulation of multiple gene modules. The genes involved in Trp metabolism of DPUL\u2010S164 was found to include genes encoding aromatic amino acid transaminases (\n AraT<\/jats:italic>\n ), genes encoding lactate dehydrogenase (ldh or ldhA) and genes encoding aldehyde dehydrogenase (ALD). During the process of DPUL\u2010S164 metabolizing Trp to produce ILA, I3C, or IAA, the key candidate genes for\n AraT<\/jats:italic>\n ,\n ldh<\/jats:italic>\n ,\n ldhA<\/jats:italic>\n , and\n ALD<\/jats:italic>\n were significantly up\u2010regulated over 3\u201324\u2009h. In addition, this study identified\n gene0696<\/jats:italic>\n and\n gene0697<\/jats:italic>\n as a functional metabolic gene cluster in DPUL\u2010S164 and involved in the regulation of its metabolic Trp\u2010producing ILA pathway, which may be involved in the regulation of Trp metabolism as a key candidate gene cluster.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n This discovery not only provides a new theoretical basis for elucidating the molecular mechanism of Trp metabolism in\n Lactobacillus<\/jats:italic>\n , but also lays an important foundation for the subsequent metabolic engineering and synthetic biology research by identifying the core functional genes. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71143","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:03:02Z","timestamp":1792040582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Elucidating the molecular mechanism of tryptophan\u2010derived indole metabolite biosynthesis in\n \n Lactiplantibacillus plantarum<\/i>\n DPUL\n <\/scp>\n \u2010\n S164<\/scp>"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4448-8941","authenticated-orcid":false,"given":"Arong","family":"Wang","sequence":"first","affiliation":[{"name":"School of Food and Bioengineering Xihua University Chengdu P. R. China"},{"name":"Food Microbiology Key Laboratory of Sichuan Province and Chongqing Key Laboratory of Speciality Food Co\u2010Built by Sichuan and Chongqing Xihua University Chengdu P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9356-753X","authenticated-orcid":false,"given":"Guangqing","family":"Mu","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology Dalian Polytechnic University Dalian P. R. China"},{"name":"Dalian Probiotics Function Research Key Laboratory Dalian Polytechnic University Dalian P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlu","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Food and Biological Engineering Beijing Vocational College of Agriculture Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Tuo","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology Dalian Polytechnic University Dalian P. R. China"},{"name":"Dalian Probiotics Function Research Key Laboratory Dalian Polytechnic University Dalian P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2000047117"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2023.04.015"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c05764"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2022.08.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2024.101798"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.05.030"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-022-01408-7"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.01.047"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-019-0689-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-017-0271-9"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.06.007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.769501"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1154346"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05470-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c06183"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.26599\/FSHW.2024.9250105"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2023.105435"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24661"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00970-4"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90293-2"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2022.2044723"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7090340"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.93"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1996.tb04331.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2026.105710"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c08616"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.08.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah5825"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.02.022"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71143","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.71143","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:03:03Z","timestamp":1792040583000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.71143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":29,"alternative-id":["10.1002\/jsfa.71143"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.71143","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"2026-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jsfa.71143"}}