{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:50:36Z","timestamp":1791532236051,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Obesity induced by long\u2010term high\u2010fat diet (HFD) consumption is closely associated with metabolic disorders and gut microbiota dysbiosis. Although plant\u2010derived extracts show anti\u2010obesity potential, microbiota\u2010dependent mechanisms and causal relevance remain incompletely understood.\n                      <jats:italic>Astragalus sinicus<\/jats:italic>\n                      L. is a traditional medicinal and dietary plant, yet the metabolic effects of its aqueous extract have not been systematically evaluated. This study investigated the anti\u2010obesity effects of an aqueous extract of\n                      <jats:italic>Astragalus sinicus<\/jats:italic>\n                      (ASAE) and determined whether gut microbiota remodeling mediates its metabolic benefits. HFD\u2010induced obese mice were treated with ASAE and subjected to comprehensive metabolic phenotyping. The chemical composition of ASAE was characterized by ultra\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight mass spectrometry. Gut microbiota composition, fecal metabolomic profiles, and adipose tissue transcriptomes were analyzed using 16S rRNA gene sequencing, untargeted metabolomics, and RNA sequencing, respectively. Fecal microbiota transplantation was performed to assess microbiota\u2010dependent causality.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>ASAE significantly improved glucose tolerance, insulin sensitivity, dyslipidemia, and hepatic steatosis in HFD\u2010fed mice without affecting food intake. ASAE markedly reshaped gut microbiota structure and fecal metabolomic profiles, particularly regulating steroid\u2010 and lipid\u2010related metabolites. Adipose transcriptomic analysis revealed that ASAE reversed HFD\u2010induced transcriptional programs associated with immune activation and lipid metabolic dysfunction. Importantly, transplantation of microbiota from ASAE\u2010treated donors recapitulated these metabolic improvements in obese recipient mice, demonstrating a microbiota\u2010dependent effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>ASAE alleviates HFD\u2010induced obesity through gut microbiota\u2010mediated mechanisms, providing causal evidence linking microbial remodeling to host metabolic improvement. These findings highlight ASAE as a promising microbiota\u2010targeted intervention for obesity. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71149","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:20:04Z","timestamp":1791530404000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary aqueous extract of\n                    <i>Astragalus sinicus<\/i>\n                    alleviates obesity by reshaping gut microbiota and metabolism"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3126-7066","authenticated-orcid":false,"given":"Yunfeng","family":"Liu","sequence":"first","affiliation":[{"name":"College of Life Sciences Anhui Normal University  Wuhu 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