{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:40:17Z","timestamp":1792431617094,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Cancer remains a major health concern worldwide, highlighting the need for safer and affordable treatments from natural sources. This study evaluated the antioxidant, antibacterial and anticancer potential of different solvent fractions of\n                      <jats:italic>Lablab purpureus<\/jats:italic>\n                      seed coat using\n                      <jats:italic>in vitro<\/jats:italic>\n                      ,\n                      <jats:italic>in vivo<\/jats:italic>\n                      and\n                      <jats:italic>in silico<\/jats:italic>\n                      approaches. Methanol, ethyl acetate, dichloromethane and\n                      <jats:italic>n<\/jats:italic>\n                      \u2010hexane extracts were subjected to phytochemical screening, antioxidant assays, cytotoxicity testing against HeLa cells, antibacterial evaluation, molecular docking and molecular dynamics studies targeting estrogen receptor alpha.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Among the tested extracts, the methanolic fraction showed the strongest antioxidant activity, with half\u2010maximal inhibitory concentration values of 86.83\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 67.56\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      in the 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (i.e. DPPH) and 2,2\u00b4\u2010azino\u2010bis(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) (i.e. ABTS) assays, respectively. This activity was consistent with its high phenolic and flavonoid contents. In a carbon tetrachloride\u2010induced oxidative stress mouse model, the methanolic extract significantly improved serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase and catalase levels. It also demonstrated strong cytotoxic activity against HeLa cells, reducing cell viability by more than 95%. The\n                      <jats:italic>n<\/jats:italic>\n                      \u2010hexane extract showed notable antibacterial activity, particularly against\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      , with a zone of inhibition of 32\u2009mm. Gas chromatography\u2010tandem mass spectrometry analysis of the methanolic crude extract identified 76 compounds, among which\n                      <jats:italic>N<\/jats:italic>\n                      \u2010benzylisatoic anhydride and pallensin showed strong binding affinity toward estrogen receptor alpha, with docking scores comparable to doxorubicin. Pallensin also exhibited favorable electronic properties, whereas molecular dynamics, molecular mechanics\/generalized born surface area and principal component analyses confirmed stable ligand\u2010receptor interactions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Overall, the findings indicate that\n                      <jats:italic>L. purpureus<\/jats:italic>\n                      seed coat is a promising source of bioactive compounds with antioxidant, antibacterial and anticancer activities. \u00a9 2026 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71151","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:08:50Z","timestamp":1792429730000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolite profiling and pharmacological evaluation of\n                    <i>Lablab purpureus<\/i>\n                    seed coat: a rich source of antioxidant, antibacterial and anticancer metabolites"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4361-5481","authenticated-orcid":false,"given":"Md Rabiul","family":"Hossain","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmaceutical Sciences University of Science and Technology Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rony","family":"Shill","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmaceutical 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