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However, free lipases suffer from poor reusability and difficult product separation. In this study,\n                      <jats:italic>Rhizomucor miehei<\/jats:italic>\n                      lipase (RML) was immobilized on chitosan microspheres (CS) to prepare a CS\u2010supported catalyst (CS\u2010G\u2010R) for the glycerolysis of glyceryl trioctanoate (TCG) with glycerol (Gly) to produce glyceryl monooctanoate (MCG) and glyceryl dioctanoate (DCG).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Characterization confirmed the successful immobilization and high dispersion of RML on the CS support. The CS\u2010G\u2010R catalyst prepared with 600\u2009\u03bcL of RML showed the best catalytic performance, achieving 76.72% TCG conversion with MCG and DCG selectivities of 38.73% and 37.26%, respectively. Molecular docking revealed strong binding between the open\u2010form RML structure (4TGL) and TCG, whereas Gly interacted weakly outside the catalytic center. Molecular dynamics simulations further confirmed the stability of the 4TGL\u2013TCG complex. Based on experimental and computational results, a glycerolysis mechanism involving acylation, deacylation, and a possible DCG\u2010mediated pathway for MCG formation was proposed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>CS\u2010G\u2010R exhibited effective catalytic activity toward TCG glycerolysis, while molecular simulations provided insights into substrate recognition and catalytic pathways. This study offers an effective strategy for developing immobilized lipases for the production of food emulsifiers and functional lipids. \u00a9 2026 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.71153","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:48:17Z","timestamp":1791521297000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glycerolysis of glyceryl trioctanoate for the preparation of glyceryl monooctanoate and dioctanoate catalyzed by chitosan microsphere\u2010immobilized\n                    <i>Rhizomucor miehei<\/i>\n                    lipase"],"prefix":"10.1002","author":[{"given":"Guoqiang","family":"Yu","sequence":"first","affiliation":[{"name":"School of Food Science and Engineering Jiangxi Agricultural University  Nanchang 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