{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:46:39Z","timestamp":1792035999170,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Postmenopausal osteoporosis is associated with disturbed calcium homeostasis and renal calcium handling. Although\n Cerasus humilis<\/jats:italic>\n proanthocyanidins (PA) show anti\u2010osteoporotic activity, their effects on renal calcium metabolism remain unclear. We hypothesized that\n C. humilis<\/jats:italic>\n PA could alleviate ovariectomy (OVX)\u2010induced bone loss by improving renal calcium reabsorption.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Compared with untreated OVX rats, 12\u2009weeks of PA treatment increased bone mineral density from 125.5 to 232.8\u2009mg\u2009cm\n \u22123<\/jats:sup>\n and improved trabecular microarchitecture (\n P<\/jats:italic>\n \u2009<\u20090.05). PA reduced\n \u03b2<\/jats:italic>\n \u2010C\u2010terminal telopeptide and tartrate\u2010resistant acid phosphatase\u20105b levels, increased bone\u2010specific alkaline phosphatase levels, decreased 24\u2009h urinary calcium excretion, and increased apparent calcium absorption from 50.4% to 87.8% (all\n P<\/jats:italic>\n \u2009<\u20090.05). Renal histopathological alterations were attenuated, with increased renal bone morphogenetic protein\u20107 and urinary Klotho levels and decreased serum fibroblast growth factor 23 levels. Renal protein expression of transient receptor potential vanilloid 5 (TRPV5) and Calbindin\u2010D28k increased by 53.0% and 45.6%, respectively (\n P<\/jats:italic>\n \u2009<\u20090.05), with corresponding mRNA upregulation.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n C. humilis<\/jats:italic>\n PA alleviated OVX\u2010induced bone loss and calcium metabolic disturbances. The effects were associated with reduced urinary calcium loss and increased renal TRPV5 and Calbindin\u2010D28k expression. Renal calcium handling may therefore contribute to the anti\u2010osteoporotic effect of\n C. humilis<\/jats:italic>\n PA in OVX rats, and the potential as a dietary ingredient for bone health warrants further investigation. \u00a9 2026 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71168","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:06:50Z","timestamp":1792033610000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\n Cerasus humilis<\/i>\n <\/scp>\n proanthocyanidins alleviate osteoporosis by regulating renal calcium metabolism"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4530-1634","authenticated-orcid":false,"given":"Xinran","family":"Li","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing PR China"},{"name":"Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxia","family":"Shu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing PR China"},{"name":"Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing PR China"},{"name":"Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiran","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing PR China"},{"name":"Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing PR China"},{"name":"Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7073-1023","authenticated-orcid":false,"given":"Weidong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing PR China"},{"name":"Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.11622\/smedj.2021036","article-title":"Menopausal osteoporosis: screening, prevention and treatment","volume":"62","author":"Yong EL","year":"2021","journal-title":"Singapore Med J"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.ecl.2021.07.005","article-title":"Physiology of calcium homeostasis: an overview","volume":"50","author":"Matikainen N","year":"2021","journal-title":"Endocrinol Metab Clin N Am"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41581-024-00835-z","article-title":"Calcium signalling and transport in the kidney","volume":"20","author":"Staruschenko A","year":"2024","journal-title":"Nat Rev Nephrol"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.762130","article-title":"Calcium transport in the kidney and disease processes","volume":"12","author":"Hanna RM","year":"2022","journal-title":"Front Endocrinol"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1007\/s00467-021-05049-0","article-title":"Molecular mechanisms altering tubular calcium reabsorption","volume":"37","author":"Downie ML","year":"2022","journal-title":"Pediatr Nephrol"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.arr.2025.102776","article-title":"Crosstalk between kidney and bones: new perspective for modulating osteoporosis","volume":"109","author":"Duan Y","year":"2025","journal-title":"Ageing Res Rev"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/s41581-022-00665-x","article-title":"Regulation of FGF23 production and phosphate metabolism by bone\u2013kidney interactions","volume":"19","author":"Agoro R","year":"2023","journal-title":"Nat Rev Nephrol"},{"key":"e_1_2_10_9_1","article-title":"Role of bone morphogenetic protein\u20107 in renal fibrosis","volume":"6","author":"Li RX","year":"2015","journal-title":"Front Physiol"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2023.102795","article-title":"A rapid chemometrics model for antioxidant substance mining of Chinese dwarf cherry [Cerasus humilis (Bge.) Sok.] based on polyphenol profile and antioxidant capacity of 30 germplasms","volume":"53","author":"Zhang YS","year":"2023","journal-title":"Food Biosci"},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","DOI":"10.26599\/FSHW.2024.9250174","article-title":"Chinese dwarf cherry [Cerasus humilis (Bge.) Sok.] polyphenol extract promotes osteoblastic differentiation in MC3T3\u2010E1 cells and reduce ovariectomy\u2010induced bone loss in rats via the calcium\/AMPK signaling pathway","volume":"14","author":"Zhang YS","year":"2025","journal-title":"Food Sci Human Wellness"},{"key":"e_1_2_10_12_1","first-page":"103","article-title":"Investigation of the effects and molecular mechanisms of proanthocyanidins in improving osteoporosis via the Wnt signaling pathway","volume":"20","author":"Feng YY","year":"2023","journal-title":"J Med Mol Biol"},{"key":"e_1_2_10_13_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fbio.2023.102846","article-title":"Exploring the beneficial effects and mechanisms of Cerasus humilis (Bge.) Sok fruit for calcium supplementation and promotion","volume":"54","author":"Li H","year":"2023","journal-title":"Food Biosci"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1177\/18785093241310545","article-title":"Accumulation, extraction, purification, and antioxidant characteristics of proanthocyanidins from Chinese dwarf cherry (Cerasus humilis)","volume":"15","author":"Li P","year":"2025","journal-title":"J Berry Res"},{"key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s41365-018-0387-z","article-title":"Methodological study on endogenous calcium absorptivity using rats and 41Ca tracing","volume":"29","author":"Zhang H","year":"2018","journal-title":"Nucl Sci Tech"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.3390\/foods9081070","article-title":"The effect of the supplementation of a diet low in calcium and phosphorus with either sheep milk or cow milk on the physical and mechanical characteristics of bone using a rat model","volume":"9","author":"Burrow K","year":"2020","journal-title":"Foods"},{"key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.bonr.2016.05.006","article-title":"Effect of micro\u2010computed tomography voxel size and segmentation method on trabecular bone microstructure measures in mice","volume":"5","author":"Christiansen AB","year":"2016","journal-title":"Bone Rep"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1186\/s12891-022-05265-1","article-title":"Early effects of ovariectomy on bone microstructure, bone turnover markers and mechanical properties in rats","volume":"23","author":"Guo XM","year":"2022","journal-title":"BMC Musculoskelet Disord"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s00198-025-07422-3","article-title":"Update on the role of bone turnover markers in the diagnosis and management of osteoporosis: a consensus paper from the European Society for Clinical and Economic Aspects of osteoporosis, osteoarthritis and musculoskeletal diseases (ESCEO), International Osteoporosis Foundation (IOF), and International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)","volume":"36","author":"Bhattoa HP","year":"2025","journal-title":"Osteoporos Int"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1146\/annurev-bioeng-062117-121139","article-title":"Bone mechanical properties in healthy and diseased states","volume":"20","author":"Morgan EF","year":"2018","journal-title":"Annu Rev Biomed Eng"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","DOI":"10.3390\/nu16040478","article-title":"Calcium deficiency decreases bone mass without affecting adiposity in ovariectomized rats fed a high\u2010fat diet","volume":"16","author":"Cao JJ","year":"2024","journal-title":"Nutrients"},{"key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.mce.2017.11.011","article-title":"Sex steroids and the kidney: role in renal calcium and phosphate handling","volume":"465","author":"Khalil R","year":"2018","journal-title":"Mol Cell Endocrinol"},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1053\/j.ajkd.2023.04.017","article-title":"Calcium and phosphate disorders: core curriculum 2024","volume":"83","author":"Murray SL","year":"2024","journal-title":"Am J Kidney Dis"},{"key":"e_1_2_10_24_1","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2023.1177829","article-title":"Pathophysiology of bone disease in chronic kidney disease: from basics to renal osteodystrophy and osteoporosis","volume":"14","author":"Aguilar A","year":"2023","journal-title":"Front Physiol"},{"key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"e970","DOI":"10.1210\/clinem\/dgae374","article-title":"Female reproductive factors, exogenous hormone use, and incident chronic kidney disease and end\u2010stage renal disease","volume":"110","author":"Han WW","year":"2025","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1080\/13880209.2024.2409772","article-title":"Proanthocyanidin offers protection against diabetic nephropathy: elucidation of its mechanism of action using animal models","volume":"62","author":"Xie D","year":"2024","journal-title":"Pharm Biol"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1007\/s00204-023-03455-x","article-title":"A mixture of 13 pesticides, contaminants, and food additives below individual NOAELs produces histopathological and organ weight changes in rats","volume":"97","author":"Dinca V","year":"2023","journal-title":"Arch Toxicol"},{"key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.6065\/apem.2019.24.4.213","article-title":"Skeletal mineralization: mechanisms and diseases","volume":"24","author":"Michigami T","year":"2019","journal-title":"Ann Pediatr Endocrinol Metab"},{"key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1146\/annurev-physiol-030212-183727","article-title":"Fibroblast growth factor 23 and klotho: physiology and pathophysiology of an endocrine network of mineral metabolism","volume":"75","author":"Hu MC","year":"2013","journal-title":"Annu Rev Physiol"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.bbrc.2022.06.101","article-title":"Exogenous BMP7 administration attenuated vascular calcification and improved bone disorders in chronic uremic rats","volume":"621","author":"Lee CT","year":"2022","journal-title":"Biochem Biophys Res Commun"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","DOI":"10.1111\/apha.13959","article-title":"Effects of parathyroid hormone on renal tubular calcium and phosphate handling","volume":"238","author":"Alexander RT","year":"2023","journal-title":"Acta Physiol (Oxf)"},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1186\/s12967-023-04182-8","article-title":"Alterations in the microenvironment and the effects produced of TRPV5 in osteoporosis","volume":"21","author":"Luo ZH","year":"2023","journal-title":"J Transl Med"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"7396","DOI":"10.3168\/jds.2022-23218","article-title":"Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5","volume":"106","author":"Zhang J","year":"2023","journal-title":"J Dairy Sci"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71168","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.71168","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.71168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:06:57Z","timestamp":1792033617000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.71168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":32,"alternative-id":["10.1002\/jsfa.71168"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.71168","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"2026-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jsfa.71168"}}