{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:45:48Z","timestamp":1792129548234,"version":"4.4.0"},"reference-count":68,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Peppermint (\n Mentha piperita<\/jats:italic>\n L.) essential oil (MPEO) is recognized for its broad\u2010spectrum antimicrobial properties and potential applications in food preservation. This study aimed to determine whether the bioactivity of MPEO is attributable to (\n R<\/jats:italic>\n )\u2010(+)\u2010limonene or whether the complete essential oil provides higher efficacy due to interactions among multiple constituents.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n MPEO was characterized using GC\u2013MS and evaluated for antimicrobial activity\n in vitro<\/jats:italic>\n against Gram\u2010positive and Gram\u2010negative bacteria and yeasts, and\n in situ<\/jats:italic>\n in the vapor phase on strawberries, plums, beetroot, and tomatoes. Antibiofilm activity against\n Salmonella enterica<\/jats:italic>\n was assessed using crystal violet assay and MALDI\u2010TOF MS, while insecticidal activity was tested against\n Callosobruchus maculatus<\/jats:italic>\n and\n Megabruchidius dorsalis<\/jats:italic>\n . GC\u2013MS revealed a menthol chemotype dominated by menthol (54.2%), with isomenthone (8.4%), isomenthol acetate (5.7%), 1,8\u2010cineole (5.7%), linalool (3.1%),\n \u03b2<\/jats:italic>\n \u2010caryophyllene (3.5%), limonene (2.7%), and\n \u03b1<\/jats:italic>\n \u2010pinene (1.5%). MPEO generally produced larger inhibition zones and lower minimum inhibitory concentration values than (\n R<\/jats:italic>\n )\u2010(+)\u2010limonene, and stronger\n in situ<\/jats:italic>\n growth suppression. Antibiofilm activity was demonstrated in both assays. In insecticidal tests, limonene was more effective than MPEO at 6.25\u201350%, while both treatments were nearly fully effective at 100%.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n MPEO showed greater overall antimicrobial efficacy than (\n R<\/jats:italic>\n )\u2010(+)\u2010limonene, supporting the importance of interactions among its constituents. Its combined antimicrobial, antibiofilm, and insecticidal activities indicate potential for use as a natural bio\u2010preservative and in integrated pest management. \u00a9 2026 The Author(s).\n Journal of the Science of Food and Agriculture<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.71172","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:01:38Z","timestamp":1792126898000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling chemical composition of peppermint oil and its bioactivities compared with (\n R<\/i>\n )\u2010(+)\u2010limonene: antimicrobial, antibiofilm, and insecticidal perspectives"],"prefix":"10.1002","author":[{"given":"Miroslava","family":"Ka\u010d\u00e1niov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Horticulture, Faculty of Horticulture and Landscape Engineering Slovak University of Agriculture Nitra Slovakia"},{"name":"School of Medical and Health Sciences VIZJA University Warszawa Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8535-0533","authenticated-orcid":false,"given":"Stefania","family":"Garzoli","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technologies of Drug Sapienza University Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhang","family":"Qiao","sequence":"additional","affiliation":[{"name":"Institute of Horticulture, Faculty of Horticulture and Landscape Engineering Slovak University of Agriculture Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2087-7267","authenticated-orcid":false,"given":"Zhaojun","family":"Ban","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology of Farm Products, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing School of Biological and Chemical Engineering, Zhejiang University of Science and Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1242-3866","authenticated-orcid":false,"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro\u2010Products Postharvest Handling of Ministry of Agriculture and Rural Affairs College of Biosystems Engineering and Food Science, Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Lou","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology of Farm Products, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing School of Biological and Chemical Engineering, Zhejiang University of Science and Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel Horacio","family":"Elizondo\u2010Luevano","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Sciences, Biomolecular Innovation Group, Faculty of Agronomy Universidad Aut\u00f3noma de Nuevo Le\u00f3n Nuevo Le\u00f3n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8123-4743","authenticated-orcid":false,"given":"Wissem","family":"Mnif","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Science, University of Bisha Bisha Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6482-527X","authenticated-orcid":false,"given":"Alessandro","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and Environment University of Pisa Pisa Italy"},{"name":"Department of Pharmacy University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Ben Saad","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Plant Improvement Centre of Biotechnology of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anis","family":"Ben Hsouna","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Plant Improvement Centre of Biotechnology of Sfax Sfax Tunisia"},{"name":"Department of Environmental Sciences and Nutrition, Higher Institute of Applied Sciences and Technology of Mahdia University of Monastir Monastir 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