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Despite this, there is not a rapid, sensitive and accurate analytical method in goat milk for rapid screening or monitoring purposes.<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:styled-content style=\"fixed-case\">RESULTS<\/jats:styled-content><\/jats:title><jats:p>One homologous and five heterologous tracers were designed and compared for fluorescence polarization immunoassay (<jats:styled-content style=\"fixed-case\">FPIA<\/jats:styled-content>) optimization. Based on the combination of a heterologous tracer (<jats:styled-content style=\"fixed-case\">PAZ\u2010FITC<\/jats:styled-content>, synthesized with pazufloxacin and <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>) and the antibody against clinafloxacin, a highly sensitive <jats:styled-content style=\"fixed-case\">FPIA<\/jats:styled-content> was established for the detection of clinafloxacin residue in goat milk for the first time. The <jats:styled-content style=\"fixed-case\">IC<jats:sub>50<\/jats:sub><\/jats:styled-content> value was 29.3 \u00b5g L<jats:sup>\u22121<\/jats:sup> for clinafloxacin in the heterologous format \u2013 six times lower than that of the combination of the homologous tracers and the antibody. The recoveries ranged from 86.8% to 104.5%, with the relative standard deviation ranging from 4.1% to 7.2%. Validation by high\u2010performance liquid chromatography (<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>) confirmed that the results obtained from the proposed <jats:styled-content style=\"fixed-case\">FPIA<\/jats:styled-content> were in agreement with those of <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:styled-content style=\"fixed-case\">CONCLUSION<\/jats:styled-content><\/jats:title><jats:p>This proposed heterologous strategy for enhanced <jats:styled-content style=\"fixed-case\">FPIA<\/jats:styled-content> is sensitive and rapid enough for the high\u2010throughput detection of clinafloxacin residue in goat milk. \u00a9 2015 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.7228","type":"journal-article","created":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T07:28:33Z","timestamp":1429774113000},"page":"1341-1346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk"],"prefix":"10.1002","volume":"96","author":[{"given":"Jiahong","family":"Chen","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Food Quality and Safety\/Guangdong Provincial Engineering and Technique Research Centre of Food Safety Detection and Risk Assessment South China Agricultural University  Guangzhou 510642 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilya A","family":"Shanin","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry MV Lomonosov Moscow State University  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