{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:23:49Z","timestamp":1784168629574,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,5,29]],"date-time":"2015-05-29T00:00:00Z","timestamp":1432857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["301\/11\/0767"],"award-info":[{"award-number":["301\/11\/0767"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youth and Sports","doi-asserted-by":"publisher","award":["LD14096"],"award-info":[{"award-number":["LD14096"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000921","name":"COST","doi-asserted-by":"crossref","award":["BM1203"],"award-info":[{"award-number":["BM1203"]}],"id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Feasibility Programme I of the Czech Republic","award":["LO1208"],"award-info":[{"award-number":["LO1208"]}]},{"name":"Structural Funds of the European Union","award":["CZ.1.05\/2.1.00\/03.0100"],"award-info":[{"award-number":["CZ.1.05\/2.1.00\/03.0100"]}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Quercetin\u20103\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucopyranoside (isoquercitrin) and quercetin\u20103\u2010<jats:italic>O<\/jats:italic>\u2010rutinoside (rutin) are common components of a normal human diet and are increasingly used in food supplements. Here their effect on mutagenesis and immunity is shown.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The <jats:italic>in vitro<\/jats:italic> (anti)mutagenic potential was compared with that of quercetin using the Ames test in <jats:italic>Salmonella typhimurium<\/jats:italic> His<jats:sup>\u2212<\/jats:sup> strains <jats:styled-content style=\"fixed-case\">TA100<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">TA98<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TA102<\/jats:styled-content>. Isoquercitrin only slightly increased the number of revertants, while rutin was totally non\u2010mutagenic. On the other hand, all compounds displayed dose\u2010dependent protective activity against <jats:styled-content style=\"fixed-case\">H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub><\/jats:styled-content>\u2010 and <jats:italic>tert<\/jats:italic>\u2010butyl hydroperoxide\u2010induced oxidative damage to the <jats:styled-content style=\"fixed-case\">TA102<\/jats:styled-content> strain and at 75\u2009\u00b5mol\u2009L<jats:sup>\u22121<\/jats:sup> inhibited <jats:styled-content style=\"fixed-case\">H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub><\/jats:styled-content>\/Fe<jats:sup>2+<\/jats:sup>\u2010induced formation of the open circular and linear forms of the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> plasmid <jats:styled-content style=\"fixed-case\">pBSIISK<\/jats:styled-content>(\u2212). In mice, none of the flavonols (0.86\u2009\u00b5mol\u2009day<jats:sup>\u22121<\/jats:sup>, 34 days) induced harmful effects. In immunized animals, all compounds enhanced <jats:italic>ex vivo<\/jats:italic> B cell proliferation; quercetin stimulated lymphocyte basal proliferation and increased the number of <jats:styled-content style=\"fixed-case\">IgM<\/jats:styled-content>\u2010producing lymphocytes. Rutin promoted <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content> cytotoxic activity, supported T cells and enhanced gut epithelium renewal. No effect on <jats:styled-content style=\"fixed-case\">IgG<\/jats:styled-content>\u2010forming cells was found.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Isoquercitrin displayed negligible and rutin no mutagenicity, but both showed significant antimutagenic and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010protective effects against oxidative damage. <jats:italic>In vivo<\/jats:italic>, they supported the readiness of the immune system for specific humoral immune response. \u00a9 2015 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.7251","type":"journal-article","created":{"date-parts":[[2015,5,9]],"date-time":"2015-05-09T07:39:34Z","timestamp":1431157174000},"page":"1492-1499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["(Anti)mutagenic and immunomodulatory properties of quercetin glycosides"],"prefix":"10.1002","volume":"96","author":[{"given":"Kate\u0159ina","family":"Valentov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Microbiology Czech Academy of Sciences  V\u00edde\u0148sk\u00e1 1083 CZ\u2010142 20 Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"\u0160\u00edma","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Czech Academy of Sciences  V\u00edde\u0148sk\u00e1 1083 CZ\u2010142 20 Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Rybkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science University of Ostrava  Chittussiho 10 CZ\u2010710 00 Ostrava Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"K\u0159\u00ed\u017ean","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Czech Academy of Sciences  V\u00edde\u0148sk\u00e1 1083 CZ\u2010142 20 Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate\u0159ina","family":"Malachov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science University of Ostrava  Chittussiho 10 CZ\u2010710 00 Ostrava Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladim\u00edr","family":"K\u0159en","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Czech Academy of Sciences  V\u00edde\u0148sk\u00e1 1083 CZ\u2010142 20 Prague 4 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu2111106"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152012802650048"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2014.03.018"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2007.05.015"},{"issue":"591356","key":"e_1_2_6_6_1","article-title":"Quercetin and cancer chemoprevention","volume":"2011","author":"Gibellini L","year":"2011","journal-title":"Evid Based Compl Altern Med"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.2013.805744"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2011.08.029"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(75)90046-1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(00)00064-6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.23.7445"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.2307\/2289645"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(99)00005-6"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.9.3165-3170.1996"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/208858a0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2007.04.007"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20487"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules17055255"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.01.028"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2797(99)00139-8"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2006.12.020"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.105064"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.03.009"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.06.029"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(90)90060-F"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules17032335"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.07.005"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.08.010"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.03.008"},{"key":"e_1_2_6_30_1","first-page":"991","article-title":"Isoquercitrin provides better bioavailability than quercetin: comparison of quercetin metabolites in body tissue and brain sections after six days administration of isoquercitrin and quercetin","volume":"67","author":"Paulke A","year":"2012","journal-title":"Pharmazie"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(03)00054-X"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2006.06.032"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(03)00114-1"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijp.2007.19.26"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007685909018"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000711451000053X"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.06.019"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.2820"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-010-1879-0"},{"key":"e_1_2_6_40_1","unstructured":"KieferD Quercetin: uses and risks. [Online]. Available:http:\/\/www.webmd.com\/vitamins\u2010and\u2010supplements\/quercetin\u2010uses\u2010and\u2010risks[30 April2015]."},{"key":"e_1_2_6_41_1","first-page":"163","article-title":"Stimulation of ileal epithelium growth and regeneration by dietary nucleotide extracts","volume":"47","author":"Sl\u00ed\u017eov\u00e1 D","year":"2004","journal-title":"Acta Med"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf2029225"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2011.08.017"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03257.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00775-4"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.7251","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.7251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:12:54Z","timestamp":1760865174000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.7251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,29]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1002\/jsfa.7251"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.7251","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,29]]},"assertion":[{"value":"2015-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}