{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T00:06:06Z","timestamp":1769817966274,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,9,24]],"date-time":"2015-09-24T00:00:00Z","timestamp":1443052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In order to survive in food\u2010processing environments and cause disease, <jats:italic>Campylobacter jejuni<\/jats:italic> requires specific survival mechanisms, such as biofilms, which contribute to its transmission through the food chain to the human host and present a critical form of resistance to a wide variety of antimicrobials.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Phytochemical analysis of thyme ethanolic extract (<jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>), thyme post\u2010hydrodistillation residue (<jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>\u2010R), and olive leaf extract (<jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content>) using high\u2010performance liquid chromatography with photodiode array indicates that the major compounds in <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>\u2010R are flavone glucuronides and rosmarinic acid derivatives, and in <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> verbascoside, luteolin 7\u2010<jats:italic>O<\/jats:italic>\u2010glucoside and oleuroside. <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>\u2010R reduced <jats:italic>C. jejuni<\/jats:italic> adhesion to abiotic surfaces by up to 30% at 0.2\u201312.5 \u00b5g <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup>, with <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>\u2010R showing a greater effect. <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> from 3.125 to 200 \u00b5g <jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content><jats:sup>\u22121<\/jats:sup> reduced <jats:italic>C. jejuni<\/jats:italic> adhesion to polystyrene by 10\u201323%. On the other hand, <jats:italic>C. jejuni<\/jats:italic> adhesion to <jats:styled-content style=\"fixed-case\">PSI<\/jats:styled-content> cl1 cells was inhibited by almost 30% over a large concentration range of these extracts.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Our findings suggest that <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>, the agro\u2010food waste material <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>\u2010R, and the by\u2010product <jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content> represent sources of bioactive phytochemicals that are effective at low concentrations and can be used as therapeutic agents to prevent bacterial adhesion. \u00a9 2015 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.7391","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T04:08:12Z","timestamp":1440475692000},"page":"2723-2730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Anti\u2010adhesion activity of thyme (<i>Thymus vulgaris<\/i> L.) extract, thyme post\u2010distillation waste, and olive (<i>Olea europea<\/i> L.) leaf extract against <i>Campylobacter jejuni<\/i> on polystyrene and intestine epithelial cells"],"prefix":"10.1002","volume":"96","author":[{"given":"Maja","family":"\u0160iki\u0107 Poga\u010dar","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, Biotechnical Faculty University of Ljubljana  Ljubljana 1000 Slovenia"},{"name":"Medical Faculty University of Maribor  Maribor 2000 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Klan\u010dnik","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Biotechnical Faculty University of Ljubljana  Ljubljana 1000 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franz","family":"Bucar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Institute of Pharmaceutical Sciences Karl\u2010Franzens University of Graz  Graz 8010 Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toma\u017e","family":"Langerholc","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Science University of Maribor  2311 Ho\u010de Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Smole Mo\u017eina","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Biotechnical Faculty University of Ljubljana  Ljubljana 1000 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2764"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2012.1298"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2012.2598"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.58"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01878-09"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-7847.95849"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3384"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2805-y"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2013.03.024"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2013.06.011"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.4.627"},{"key":"e_1_2_7_13_1","first-page":"177","article-title":"Classical and molecular identification of thermotolerant Campylobacter from poultry meat","volume":"40","author":"Zorman T","year":"2002","journal-title":"Food Technol Biotechnol"},{"key":"e_1_2_7_14_1","first-page":"94","article-title":"Establishment of a functional cell culture model of the pig small intestine","volume":"23","author":"Gradi\u0161nik L","year":"2006","journal-title":"ALTEX"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.11.6043"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6621"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"6","author":"Mossmann T","year":"1983","journal-title":"J Immunol Methods"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0301"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88458-0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf3027759"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/np010636j"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2401"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/12051153"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ifset.2012.11.008"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2005.07.042"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0091"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.10.006"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2006.04.004"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.11.035"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6017"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0051800","article-title":"Anti\u2010Campylobacter activities and resistance mechanisms of natural phenolic compounds in Campylobacter","volume":"7","author":"Klan\u010dnik A","year":"2012","journal-title":"PLOS ONE"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000327250"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03912"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2011.09.027"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.7391","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.7391","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:55:46Z","timestamp":1760867746000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.7391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,24]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1002\/jsfa.7391"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.7391","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,24]]},"assertion":[{"value":"2015-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}