{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:34:09Z","timestamp":1790483649834,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Centro Universit\u00e1rio UNIVATES for financial support and FAPERGS (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul) for fellowship to EMS"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In the current agricultural model, the massive use of chemical fertilizer causes environmental and economic losses. Inoculation of plant\u2010growth\u2010promoting (<jats:styled-content style=\"fixed-case\">PGP<\/jats:styled-content>) nitrogen\u2010fixing bacteria is an alternative to the use of nitrogen, phosphorus and potassium fertilizers. In this study, rhizobia strains isolated from common bean (<jats:italic>Phaseolus vulgaris<\/jats:italic>) root nodules were evaluated in an effort to identify an efficient nitrogen\u2010fixing rhizobia strain able to improve bean germination and growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Common bean plants were collected from seven sites in southern Brazil, and 210 native rhizobia isolates were obtained. Evaluation of <jats:styled-content style=\"fixed-case\">PGP<\/jats:styled-content> traits showed that most of the rhizobia isolates were non\u2010siderophore producers and weak indolic compounds producers. Under laboratory conditions, rhizobia isolates <jats:styled-content style=\"fixed-case\">E15<\/jats:styled-content> (<jats:italic>Rhizobium leguminosarum<\/jats:italic>) and <jats:styled-content style=\"fixed-case\">L5<\/jats:styled-content> (<jats:italic>Rhizobium radiobacter<\/jats:italic>) increase germination percentage, length, and dry weight of common bean and arugula (<jats:italic>Eruca sativa<\/jats:italic>) seedlings. Under greenhouse conditions, common bean plants inoculated with the rhizobia isolates <jats:styled-content style=\"fixed-case\">VC28<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">L15<\/jats:styled-content> (both <jats:italic>Rhizobium fabae<\/jats:italic>) presented the highest nodule number and shoot dry matter, while <jats:styled-content style=\"fixed-case\">VC28<\/jats:styled-content> also presented the highest values of shoot nitrogen and potassium. Isolate <jats:styled-content style=\"fixed-case\">L17<\/jats:styled-content> presented highly effective N fixation, even with reduced nodulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These new rhizobia isolates are attractive <jats:styled-content style=\"fixed-case\">PGP<\/jats:styled-content> alternatives to chemical fertilizers. \u00a9 2015 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.7527","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T03:14:46Z","timestamp":1447384486000},"page":"3446-3453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Inoculation of new rhizobial isolates improve nutrient uptake and growth of bean (<i>Phaseolus vulgaris<\/i>) and arugula (<i>Eruca sativa<\/i>)"],"prefix":"10.1002","volume":"96","author":[{"given":"Eduardo M","family":"de Souza","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biotecnologia (PPGBiotec) Centro Universit\u00e1rio UNIVATES  Lajeado RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor L","family":"Bassani","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Microbiologia Agr\u00edcola e do Ambiente (PPGMAA) Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul A","family":"Sperotto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biotecnologia (PPGBiotec) Centro Universit\u00e1rio UNIVATES  Lajeado RS Brazil"},{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade (CCBS) Centro Universit\u00e1rio UNIVATES  Lajeado RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille E","family":"Granada","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biotecnologia (PPGBiotec) Centro Universit\u00e1rio UNIVATES  Lajeado RS Brazil"},{"name":"Centro de Gest\u00e3o Organizacional (CGO) Centro Universit\u00e1rio UNIVATES  Lajeado RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Global Estimates of Gaseous Emissions of NH3, NO and N2O from Agricultural Land","author":"International Fertilizer Industry Association\/Food and Agriculture Organization of the United Nations","year":"2001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/472159a"},{"key":"e_1_2_6_4_1","unstructured":"Food and Agriculture Organization of the United Nations World Food Situation: FAO Food Price Index. 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