{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:43:48Z","timestamp":1790311428561,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T00:00:00Z","timestamp":1462838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31271844"],"award-info":[{"award-number":["31271844"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Seafood is regarded as a high\u2010purine food that may induce gout, which has attracted extensive attention concerning its safety. Therefore, the aim of this study was to develop a simple and reliable method to determine the purine content in seafood and its change during storage to offer consumers healthy diet information.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Chromatographic separation was carried out using Waters Atlantis <jats:styled-content style=\"fixed-case\">dC<jats:sub>18<\/jats:sub><\/jats:styled-content> column, and potassium phosphate monobasic solution (0.02 mol L<jats:sup>\u22121<\/jats:sup>, <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 3.6) as a mobile phase. The average recovery yields of four purines were 91.5\u2013105.0%, and relative standard deviation values were around 1.8\u20136.5%. Shrimp and snail contained higher amounts of purine than fish and bivalves; the livers and skins of fish contained higher amounts of purine than muscles; and the main purine varied depending on the type of seafood. Also, purine content of seafood changed during storage.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The purine content of seafood differed depending on species, body part and degree of freshness, which could recommend consumers a healthy diet, especially for people with hyperuricemia and gout. \u00a9 2016 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.7755","type":"journal-article","created":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T06:54:20Z","timestamp":1460703260000},"page":"520-525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Determination of four different purines and their content change in seafood by high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"97","author":[{"given":"Xin","family":"Qu","sequence":"first","affiliation":[{"name":"Food Safety Laboratory Ocean University of China  Qingdao 26600 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxin","family":"Sui","sequence":"additional","affiliation":[{"name":"Food Safety Laboratory Ocean University of China  Qingdao 26600 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasha","family":"Mi","sequence":"additional","affiliation":[{"name":"Food Safety Laboratory Ocean University of China  Qingdao 26600 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"Food Safety Laboratory Ocean University of China  Qingdao 26600 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1383\/medc.30.9.71.28246"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2004.09.002"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2006.07.015"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000150784.92944.9a"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(04)00934-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30520"},{"key":"e_1_2_6_8_1","first-page":"548","article-title":"A five years follow up study on patients with gout and hyperuricemia","volume":"27","author":"Yan S","year":"2011","journal-title":"Zhonghua Neifenmi Daixie Zazhi"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2005.07.027"},{"key":"e_1_2_6_10_1","first-page":"1","volume-title":"Disorders of Purine and Pyrimidine Metabolism","author":"Seegmiller JE","year":"1979"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20761"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.bor.0000209434.82096.1f"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.mpmed.2006.07.012"},{"key":"e_1_2_6_14_1","first-page":"1438","article-title":"The application of dietary guidance in gout treatment","volume":"12","author":"Li F","year":"2010","journal-title":"Chronic Dis J"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6198"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/15257771003741372"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1094\/ASBCJ-2015-0409-01"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1197"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(01)01570-7"},{"key":"e_1_2_6_20_1","first-page":"34","article-title":"Determination of purine content in soybean by HPLC","volume":"4","author":"Liu S","year":"2009","journal-title":"J Anhui Agric Univ"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/15257770802138681"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.04.033"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.08.059"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa035700"},{"key":"e_1_2_6_25_1","first-page":"1859","article-title":"Dietary and lifestyle changes associated with high prevalence of hyperuricemia and gout in the Shandong coastal cities of Eastern China","volume":"35","author":"Miao Z","year":"2008","journal-title":"J Rheumatol"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/106.3.435"},{"key":"e_1_2_6_27_1","first-page":"153","article-title":"Contents of purine bases in fishes and fish products","volume":"34","author":"Takako S","year":"1981","journal-title":"Japan Soc Nutr Food Sci"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0889-1575(88)90016-6"},{"key":"e_1_2_6_29_1","first-page":"23","article-title":"The contents of purine in common animal foods in China","volume":"1","author":"Pan H","year":"2012","journal-title":"Acta Nutr Sin"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b13-00967"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(02)00173-5"},{"key":"e_1_2_6_32_1","first-page":"41","article-title":"Determination of purines in food by high performance liquid chromatography","volume":"44","author":"Qu X","year":"2014","journal-title":"Periodical Ocean Univ China"},{"key":"e_1_2_6_33_1","first-page":"219","article-title":"Determination of purine compounds in beer by reversed\u2010phase ion pair chromatography","volume":"27","author":"Lin XJ","year":"2006","journal-title":"Food Sci"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/106.3.428"},{"key":"e_1_2_6_35_1","first-page":"102","article-title":"Quality changes of brined common carp (Cyprinus carpio) fillets during chilled storage and partial freezing","volume":"40","author":"Chen K","year":"2015","journal-title":"Food Sci Technol \u2013 Brazil"},{"key":"e_1_2_6_36_1","first-page":"13","article-title":"Effect of different slaughter methods on quality of Oreochromis sp. fillets","volume":"9","author":"Fang J","year":"2013","journal-title":"South China Fisheries Sci"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/009127007001000505"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.28.519"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/cg0704753"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1998.tb15760.x"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.7755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.7755","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.7755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:03:36Z","timestamp":1760879016000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.7755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,10]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1002\/jsfa.7755"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.7755","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,10]]},"assertion":[{"value":"2016-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}