{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:39:38Z","timestamp":1774967978576,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T00:00:00Z","timestamp":1502236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University Board of Research, Ahmadu Bello University Zaria, Nigeria"},{"name":"The Organization for Women in Science for the Developing World (OWSDW)","award":["3240253937"],"award-info":[{"award-number":["3240253937"]}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Some routes of transmission of <jats:italic>Escherichia coli<\/jats:italic> O157:H7 to fresh produce include contaminated irrigation water and manure polluted soils. The aim of the present study was to determine the genetic relationships of <jats:italic>E. coli<\/jats:italic> O157:H7 isolated from some produce growing region in Nigeria using enterobacterial repetitive intergenic consensus (ERIC) DNA fingerprinting analysis. A total of 440 samples comprising leafy greens, irrigation water, manure and soil were obtained from vegetable producing regions in Kano and Plateau States, Nigeria. Genes coding for the quinolone resistance\u2010determinant (gyrA) and plasmid (pCT) coding for multidrug resistance (MDR) were determined using polymerase chain reaction (PCR) in 16 isolates that showed MDR.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Cluster analysis of the ERIC\u2010PCR profiles based on band sizes revealed six main clusters from the sixteen isolates analysed. The largest cluster (cluster 3) grouped isolates from vegetables and manure at a similarity coefficient of 0.72.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The present study provides data that support the potential transmission of resistant strains of <jats:italic>E. coli<\/jats:italic> O157:H7 from vegetables and environmental sources to humans with potential public health implications, especially in developing countries. \u00a9 2017 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.8441","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T20:35:59Z","timestamp":1495571759000},"page":"80-86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Fingerprints of resistant <i>Escherichia coli<\/i> O157:H7 from vegetables and environmental samples"],"prefix":"10.1002","volume":"98","author":[{"given":"Grace Onyukwo","family":"Abakpa","sequence":"first","affiliation":[{"name":"National Biotechnology Development Agency  Abuja Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronica J","family":"Umoh","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Akwa Ibom State University  Mkpat Enin LGA Akwa Ibom State Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijam","family":"Kamaruzaman","sequence":"additional","affiliation":[{"name":"Department of Agrobio Universiti Putra  Serdang Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Ibekwe","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS\u2010US Salinity Laboratory  Riverside CA 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