{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:05Z","timestamp":1786978145734,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T00:00:00Z","timestamp":1505692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572425"],"award-info":[{"award-number":["31572425"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      During the last phase of incubation, dramatic physiological and metabolic changes occur in chick embryos, and supplies of nutrients and energy are always insufficient. This study investigated the effects of\n                      <jats:italic>in ovo<\/jats:italic>\n                      feeding (IOF) of\n                      <jats:sc>l<\/jats:sc>\n                      \u2010arginine (Arg) on the hatchability, growth performance, intestinal development and functions of posthatch broilers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The IOF of Arg increased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) the feed intake and body weight gain during 1\u201321\u2009days and 1\u201342\u2009days, and the intestinal weight of 7\u2010 and 21\u2010day\u2010old broilers, compared with non\u2010injected control and diluent\u2010injected groups. The IOF of Arg increased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) villus height (VH), ratio of VH to crypt depth (CD) and density of goblet cells, and decreased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) the CD in jejunum of 1\u2010, 7\u2010 and 21\u2010day\u2010old broilers. The IOF of Arg also increased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) the percentage of proliferating cell nuclear antigen positive cells of villus, and the mRNA expressions of mucin\u20102, claudin\u20101, zonula occludens\u20101 and \u20102 in jejunal mucosa of 21\u2010day\u2010old broilers. Meanwhile, IOF of Arg increased (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) the protein abundance of phosphorylated mechanistic target of rapamycin (mTOR), ribosomal protein S6 kinase 1 and eukaryotic initiation factor 4E binding protein 1 in jejunal mucosa.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>The IOF of Arg improved the development and barrier functions of small intestine, which might be associated with activating the mTOR pathway. In addition, the improved intestinal development might explain the improvement in feed intake and consequently the growth performance of broilers. Therefore, IOF of Arg solution could be an effective technology for regulating early nutrition supply and subsequent growth development in the poultry industry. \u00a9 2017 Society of Chemical Industry<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.8609","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T16:27:21Z","timestamp":1501777641000},"page":"1416-1425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["<i>In ovo<\/i>\n                    feeding of\n                    <scp>l<\/scp>\n                    \u2010arginine regulates intestinal barrier functions of posthatch broilers by activating the mTOR signaling pathway"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1716-8497","authenticated-orcid":false,"given":"Tian","family":"Gao","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Key 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