{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T22:10:16Z","timestamp":1774649416076,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004387","name":"Ningbo University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004387","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Steryl glycosides (SGs) are sterol conjugates found in various plants, especially in those making up human diets. It has been demonstrated that SGs have potential health benefits, and they could be used as food supplements in a variety of food matrixes. Marine microalgae are a potential resource for human food and ingredients. In this study, gas chromatography\u2013triple quadrupole mass spectrometry (GC\u2013QQQ\u2010MS) was used to characterize unknown SGs in eight microalgae belonging to different classes (<jats:italic>Isochrysis galbana<\/jats:italic> 3011, <jats:italic>Pavlova viridis<\/jats:italic>, <jats:italic>Platymonas helgolandica<\/jats:italic>, <jats:italic>Conticribra weissflogii<\/jats:italic>, <jats:italic>Thalassiosira pseudonana<\/jats:italic>, <jats:italic>Nitzschia closterium<\/jats:italic>, <jats:italic>Gymnodinium<\/jats:italic> sp., and <jats:italic>Karlodinum veneficum<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The SGs were first extracted from lyophilized algae with chloroform\u2013methanol, purified by solid\u2010phase extraction and analyzed as trimethylsilyl derivatives. Nine SGs have been identified. In particular, new SGs like occelasteryl glycoside and stellasteryl glycoside were found in <jats:italic>Gymnodinium<\/jats:italic> sp., 24\u2010methylene cholesteryl glycoside was detected in <jats:italic>P. helgolandica<\/jats:italic>, and 4,24\u2010dimethylcholestan\u20103\u2010yl glycoside was identified as the main constituent of microalga <jats:italic>K. veneficum<\/jats:italic>. The results also showed that the compositions of SGs in different microalgae varied, with a range of 5.234 to 0.036\u2009g kg<jats:sup>\u22121<\/jats:sup>, and microalga <jats:italic>P. viridis<\/jats:italic> contained the most abundant SGs.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>GC\u2013QQQ\u2010MS is a powerful tool to detect SGs with different structures from a variety of microalgae. The compositions of SGs in different microalgae varied greatly. Microalgae are a good source of highly valued SGs. \u00a9 2017 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.8629","type":"journal-article","created":{"date-parts":[[2017,8,21]],"date-time":"2017-08-21T11:32:38Z","timestamp":1503315158000},"page":"1574-1583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of steryl glycosides in marine microalgae by gas chromatography\u2013triple quadrupole mass spectrometry (GC\u2013QQQ\u2010MS)"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1256-7074","authenticated-orcid":false,"given":"Shanshan","family":"Yu","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University Chinese Ministry of Education  Ningbo China"},{"name":"Collaborative Innovation Center for Zhejiang Marine High\u2010Efficiency and Healthy Aquaculture Ningbo University  Ningbo 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jilin","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Marine Biotechnology, Ningbo University Chinese Ministry of Education  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengxu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center for Zhejiang Marine High\u2010Efficiency and Healthy Aquaculture Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Yan","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center for Zhejiang Marine High\u2010Efficiency and Healthy Aquaculture Ningbo University  Ningbo 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